国际医药卫生导报2015，Vol.21Issue(13)：1837-1839,1850,4.DOI:10.3760/cma.j.issn.1007-1245.2015.13.017
DHA对人鼻咽癌CNE2细胞增殖的抑制作用及机制研究
Effect and mechanism of DHA inhibiting proliferation of CNE2 cells in human nasopharyngeal carcinoma
詹建东 1邱前辉 1苏小妹 1许咪咪1
作者信息
- 1. 510080 广州,广东省人民医院耳鼻科广东省医学科学院
- 折叠
摘要
Abstract
Objective To study the influence of DHA on the proliferation and apoptosis of CNE2 cells in nasopharyngeal carcinoma.Methods CCK8 assay was used to investigate the influence of DHA on the proliferation of CNE2 cells.Flow eytometry (FCM) were used to examine the effect of DHA on the apoptosis of CNE2 cells.Reverse transcription polymerase chain reaction (RT-PCR) was applied to detect the mRNA expressions of Bcl-2 and Bcl-xl in CNE2 cells.Results DHA more significantly inhibited the proliferation of CNE2 cells than the control.FCM method indicated that the apoptosis of CNE2 cells was significantly higher after exposure to DHA than to the control (P ＜ 0.05).The mRNA levels of Bcl-2 and Bclxl were significantly decreased in the DHA group.Conclusions DHA can inhibit the proliferation and induce the apoptosis of CNE2 cells down-regulating the mRNA expressions of Bcl-2 and Bcl-xl.关键词
鼻咽癌/凋亡/DHA/Bcl-2/Bcl-xlKey words
Nasopharyngeal carcinoma/Apoptosis/DHA/Bcl-2/Bcl-xl引用本文复制引用
詹建东,邱前辉,苏小妹,许咪咪..DHA对人鼻咽癌CNE2细胞增殖的抑制作用及机制研究[J].国际医药卫生导报,2015,21(13):1837-1839,1850,4.
