国际医药卫生导报2015，Vol.21Issue(13)：1840-1843,4.DOI:10.3760/cma.j.issn.1007-1245.2015.13.018
小细胞肺癌患者外周血循环肿瘤细胞的检测及其临床意义
Detection of tumor cells in peripheral circulation in patients with small cell lung cancer and its clinical significance
丁凌雁 1唐朝晖 1黄洪林 1何宝桂 1康马飞2
作者信息
- 1. 425000 永州市中心医院肿瘤科
- 2. 541001 桂林,桂林医学院附属医院肿瘤内科
- 折叠
摘要
Abstract
Objective To investigate the detection of tumor cells in peripheral circulation in patients with small cell lung cancer (SCLC) and its clinical significance.Methods 80 patients with SCLC treated in the Department of Oncology at Yongzhou Central Hospital from August,2011 to August,2014 were selected as an observation group and 50 patients with benign lung disease a control group.7.5 ml peripheral blood was collected from the patients respectively.Density gradient centrifugation method and Quantitative Real-time Reverse Transcription-PCR method were used to detect the CTC expression with cytokeratin-19 positive in the two groups before and two cycles after the chemotherapy.Then the relationship between clinical features and chemical effect was analyzed.Results CTC expression was detected in 56 cases in the observation group,with a positive rate of 70％,and in no one in the control group,with a statistical difference between the two groups (P＜0.05).CTC expression correlated with clinical stages and recurrence (P＜0.05),but not with gender,age,organization type,smoking,and degree of differentiation (P＞0.05).Before the chemotherapy,CTC expression was detected in 56 cases;after two cycles chemotherapy,CTC expression was detected in 24 cases;with a statistical difference (P＜0.05).After two cycles chemotherapy,there were no statistical differences in chemotherapy effects between the evaluations of CTC numbers and Response Evaluation Criteria in Solid Tumors (x 2=0.002,P＞0.05).CTC expression correlated with the clinical efficacy (r=-0.025,P=0.041).Conclusions The positive rate of CTC correlates with the clinical stages and recurrence and negatively with chemotherapy effect.The positive rate of CTC can predict the chemotherapy effects in SCLC.关键词
小细胞肺癌/循环肿瘤细胞/qRT-PCR法/化疗疗效Key words
Small cell lung cancer/Tumor cells in peripheral circulation/Quantitative Real-time Reverse Transcription-PCR/Chemotherapy effect引用本文复制引用
丁凌雁,唐朝晖,黄洪林,何宝桂,康马飞..小细胞肺癌患者外周血循环肿瘤细胞的检测及其临床意义[J].国际医药卫生导报,2015,21(13):1840-1843,4.
