广东医学Issue(2)：178-181,4.
肝脏异维甲酸受体α调控脂代谢通路的基因组靶点分析
Genome-wide analysis of retinoid X receptor αin the lipid metabolism of mouse livers
詹琪 1聂玉强 1李瑜元1
作者信息
- 1. 广州医科大学附属广州市第一人民医院消化内科、广东省广州市消化疾病中心 510180
- 折叠
摘要
Abstract
Objective To characterize the genome targets of hepatic retinoid X receptor α( RXRα) as well as the correlation between its downstream pathways and lipid metabolism .Methods Six mice were divided into treatment group and control group (n=3), which were treated by retinoic acid 150 mg/(kg· d) or equal amounts of saline solution for 7 days, respectively.Serum lipid was detected.Hepatic DNA fractions bound by RXRαwere immunoprecipitated and sequenced .The distribution of RXRαbindings was characterized .Real-time PCR was used to evaluate the expression of RXRαtarget genes .Results Serum cholesterol and triglyceride were reduced significantly in treatment group compared to control group(P<0.05).RXRαbindings and target genes in treatment group were increased compared to control group , among which the overlapped transcriptional start site were increased the most by 58%.Genes implicated in oxidation re-duction, lipid metabolism and lipid transportation were targeted by RXRα.Among the genes associated with lipid metabo-lism in treatment group, 48%(11 out of 23) were down-regulated whereas 22%(5 out of 23) were up-regulated sig-nificantly.Conclusion RXRαbinds extensively to the mouse liver genome .It regulates the hepatic lipid metabolism at the transcriptional level .关键词
异维甲酸受体α/脂代谢/核受体/染色体免疫共沉淀测序Key words
retinoid X receptor α/lipid metabolism/nuclear receptor/chromatin immunoprecipitation sequen-cing引用本文复制引用
詹琪,聂玉强,李瑜元..肝脏异维甲酸受体α调控脂代谢通路的基因组靶点分析[J].广东医学,2015,(2):178-181,4.
