广东医学Issue(7)：981-984,4.
高同型半胱氨酸血症对 ApoE －／－鼠心脏内质网应激的影响
The effects of hyperhomocysteinemia on myocardial endoplasmic reticulum stress in ApoE-/-mice
摘要
Abstract
Objective To investigate the effects of hyperhomocysteinemia ( HHcy ) on myocardial endoplasmic reticulum stress ( ERS) in ApoE-/-mice and its possible mechanism.Methods Thirty-six 5 -week old ApoE-/-C57BL/6J mice were randomly divided into three groups, including ApoE-/-control group, ApoE-/-HHcy group and the intervention group.Twelve 5-week old normal C57BL/6J mice were served as normal group.After 20 weeks of feed-ing, the aortae were separated for HE stain and observation of aortic plaque.GRP78, CHOP and Caspase-12 mRNA lev-els were determined by qRT-PCR.The methylation status of GRP78 and CHOP were studied using the nest touch down methylation specific polymerase chain reaction ( ntMS-MSP) .Results No significant aortic plaque was observed in ei-ther normal group or ApoE-/-control group.In the ApoE-/-HHcy group, the aortic plaque areas were greater, breaking into the aortic lumina, which were alleviated in the intervention group.The mRNA levels of GRP78, CHOP and Caspase-12 in the ApoE-/-HHcy group were significantly higher than those in ApoE-/-control group(P<0.05,P<0.01), while they were significantly down-regulated in the intervention group(P<0.05,P<0.01).GRP78 and CHOP DNA methylation levels were significantly lowered in the ApoE-/-HHcy group(P<0.01,P<0.01),while significanty higher in the intervention group(P<0.05,P<0.05).Conclusion HHcy may affect Myocardial ERS via changing DNA methy-lation levels, then lead to apoptosis.Earlier folic acid and VB12 interference can effectively reduce the myocardial ERS and apoptosis.关键词
高同型半胱氨酸血症/内质网应激/葡萄糖调节蛋白78/C/EBP环磷酸腺苷反应元件结合转录因子同源蛋白/胱冬酶-12Key words
hyperhomocysteinemia/endoplasmic retculum stress/glucose-regulatedprotein78/C/EBP homolo-gousprotein/Caspase-12引用本文复制引用
周龙霞,赵丽,陈久凯,杨安宁,刘现梅,王艳华,姜怡邓,曹军..高同型半胱氨酸血症对 ApoE －／－鼠心脏内质网应激的影响[J].广东医学,2015,(7):981-984,4.基金项目
国家自然科学基金资助项目 （）
