广东医学Issue(8)：1145-1148,4.
Ⅱ型胶原蛋白酶诱导 SD 大鼠膝骨关节炎模型的建立
Establishment of SD rat model on knee osteoarthritis induced by type Ⅱ collagen enzyme
摘要
Abstract
Objective To establish the knee osteoarthritis ( OA) model using type Ⅱ collagen enzyme in SD rats.Methods Twenty SD rats were randomly divided into Group A ( model group) and Group B ( normal saline control group), in which typeⅡcollagen enzyme and saline were given by knee injection, respectively, on the 1st and 4th day. Body weight on the 1st, 3rd and 7th day, knee joint diameter on the 1st and 4th day were recorded.The subjects were sacri-ficed on the 7 th day for assessment of spleen and pancreas weights, and viscera coefficient, while the the general morpholo-gy of the knee joint tissues with HE staining was also observed.Results After modeling, body weight reduced in Group A, while it increased in Group B.The knee joint diameter was significantly larger after modeling than before modeling in Group A (P<0.01), while there was no significant difference revealed in Group B (P>0.05).Knee joint diameter in Group A was significantly larger than that in Group B after the modeling (P<0.01).Early OA was observed in Group A, while no evidence was revealed in Group B.Conclusion By using collagen typeⅡenzymes, early knee OA model can be successfully established in SD rats.Body weight, knee joint diameter, spleen and pancreas weights, organ coefficient and knee joint pathology could be applied for study of the pathophysiological features of this OA model.关键词
胶原蛋白酶/膝骨关节炎/关节软骨/模型Key words
collagen enzyme/knee osteoarthritis/articular cartilage/model引用本文复制引用
黄永明,潘建科,郭达,刘军,杨济源,洪坤豪,杨伟毅,许树柴..Ⅱ型胶原蛋白酶诱导 SD 大鼠膝骨关节炎模型的建立[J].广东医学,2015,(8):1145-1148,4.基金项目
国家自然科学基金资助项目（编号81273781，81473698），教育部高等学校博士学科点专项科研基金资助课题（博导类）（编号20124425110004），2011年广东省第一批科学事业费计划项目（编号2011B031700027），广东省财政厅“中老年退行性膝骨关节炎中医药防治管理体系”项目（编号［2014］157号），2013年度广东省中医院中医药科学技术研究专项（第二批）立项课题 （）
