广东医学Issue(9)：1309-1312,1313,5.
人骨髓间充质干细胞成骨分化相关的长链非编码RNA 表达谱特征
Expression profile of long non - coding RNAs involved in osteogenic differentiation of human marrow - derived mesenchymal stem cells
摘要
Abstract
Objective To analyze the expression profile variation of long non - coding RNA(lncRNA)during osteogenesis of human marrow - derived mesenchymal stem cells(hMSCs). Methods Osteoblast - induced differentia-tion was perfomed on hMSCs. Using Agilent lncRNA microarray,samples of hMSCs and differentiated hMSCs on the 7th , 14th and 21st day were assayed to identify the target lncRNA,which was altered more than 2 times during osteogenesis of hMSCs. The expression of lncRNA was verified by real time quantification - polymerase chain reaction(RT - PCR). Spe-cific lncRNAs related protein - coding genes using the UCSC Genome Browser combined with microarray results. Results Osteogenic characteristics were observed in induced hMSCs on the 7th ,14th and 21st day. According to microarray analy-sis,433 lncRNAs were constantly significantly up - regulated more than 2 times and 232 lncRNAs constantly significantly down - regulated more than 2 times during the osteogenesis of hMSCs. The results of RT - PCR were consistent with miRNA microarray. Analysis of nearby protein - coding genes prompted that some lncRNAs,such as lncRNA ENST00000585537. 1 and lncRNA eHIT00001595,may play important roles in osteogenesis of hMSCs. Conclusion During osteogenesis of hMSCs,lncRNA expression profiles have significantly changed,these different lncRNAs maybe closely related to the oste-ogenesis of hMSCs and these findings can also further explain the mechanism of osteogenesis of hMSCs.关键词
人骨髓间充质干细胞/成骨分化/长链非编码RNAKey words
hMSCs/osteogenesis/lncRNA引用本文复制引用
孙翔,赵建江,贾搏,韩久松,邱小玲,褚洪星,郑相淮..人骨髓间充质干细胞成骨分化相关的长链非编码RNA 表达谱特征[J].广东医学,2015,(9):1309-1312,1313,5.基金项目
广东省科技计划项目（编号2013B021800153），广州市科技惠民专项项目 （）
