广东医学Issue(9)：1314-1317,4.
人参皂苷 Rg3对人鼻咽癌 CNE -2细胞血管生成拟态及迁移能力的影响
20(R)- ginsenoside Rg3 inhibits vasculogenic mimicry and cell migration of human nasopharyngeal carcinoma CNE-2 cell line in vitro
摘要
Abstract
Objective To study the inhibitory effects of 20(R)- ginsenoside Rg3 on vasculogenic mimicry and migration of human nasopharyngeal carcinoma CNE - 2 cell line in vitro. Methods CNE - 2 cells treated with different concentrations of Rg3(0 μmol·L - 1 ,38 μmol·L - 1 ,76 μmol·L - 1 and 114 μmol·L - 1 )were assayed with transwell assay and anti - angiogenic test for testing the potential of migration and tube - like structure(TLSs)formation,respec-tively. Meanwhile,the expression of COX - 2,HIF - 1α,VEGF and Fascin1 proteins were detected by Western blotting. Results The formation of TLSs in CNE - 2 cells were significantly inhibited by Rg3 in concentration dependent manners (P < 0. 01);so was the migration ability of CNE - 2 cells(P < 0. 05). At the same time,the expression of COX - 2, HIF - 1α,VEGF and Fascin1 in CNE - 2 cells were down - regulated by Rg3. A significantly positive correlation between the expression of Fascin1 and the migration ability was observed(P < 0. 05),so was the significantly positive correlation between the expression of COX - 2,HIF - 1α and VEGF and the inhibition of the TLS formation(P < 0. 05). Conclusion Rg3 can inhibit the vasculogenic mimicry and migration of CNE - 2 cells in vitro,via the down - regulation of COX - 2, HIF - 1α,VEGF and Fascin1.关键词
人参皂苷Rg3/鼻咽癌/血管生成拟态/细胞迁移Key words
20(R)- Ginsenoside Rg3/Nasopharyngeal carcinoma/Vasculogenic mimicry/Cell migration引用本文复制引用
孔霞,尚九龙,周艳芳,王槐高,顾帝水..人参皂苷 Rg3对人鼻咽癌 CNE -2细胞血管生成拟态及迁移能力的影响[J].广东医学,2015,(9):1314-1317,4.基金项目
广东省中医药管理局建设中医药强省科研课题（编号20122082），广东省医学科研基金资助项目（编号B2011235），广东省东莞市高等院校科研计划项目（编号200910815267），广东省湛江市科技攻关项目（编号2009C3101013），广东医学院面上项目资助课题 （）
