中国组织工程研究Issue(47)：7615-7620,6.DOI:10.3969/j.issn.2095-4344.2014.47.013
纳米羟基磷灰石/细菌纤维素复合组织工程支架的细胞毒性和生物相容性
Cytotoxicity and biocompatibility of nano-hydroxyapatite/bacterial cellulose composite scaffolds
摘要
Abstract
BACKGROUND:Nano-hydroxyapatite/bacterial celulose (nHA/BC) composite scaffolds prepared by nano-bionics method exhibit a structure and performance similar to natural bone. OBJECTIVE:To study the cytotoxicity and biocompatibility of nHA/BC composite scaffolds. METHODS:(1) Acute systemic toxicity test: nHA/BC leach liquor and normal saline were respectively injected intraperitonealy into Kunming mice. After 24, 48, 72 hours, the body mass of mice was recorded. (2) Sensitization test: nHA/BC leach liquor and normal saline were respectively injected subcutaneously into the back of Japanese white rabbits. With 72 hours of injection, edema and erythema were observed, and the experiment was done again at an interval of 14 days. (3) Pyrogen test: nHA/BC leach liquor and normal saline were respectively injected into the ear vein of Japanese white rabbits, and after injection, the body temperature of rabbits were determined. (4) Hemolysis test: Diluted rabbit anti-coagulant was added into nHA/BC leach liquor, normal saline and distiled water, respectively. (5) Passage 3 bone marrow mesenchymal stem cels from rabbits were co-cultured with nHA/BC materials, and then, cel proliferation, growth and adhesion on the material surface were observed. RESULTS AND CONCLUSION: nHA/BC composite scaffold was of no acute systemic toxicity, non-alergenic, no pyrogenic and hemolytic reactions; nHA/BC had a three-dimensional porous structure that was better for growth, proliferation and adhesion of rabbit bone marrow mesenchymal stem cels. These findings indicate that the nHA/BC composite scaffold has good biocompatibility and cytocompatibility.关键词
生物材料/材料相容性/纳米羟基磷灰石/细菌纤维素/组织工程/生物相容性Key words
nanoparticles/hydroxyapatites/celulose分类
医药卫生引用本文复制引用
王立新,袁峰,万怡灶,何啸波..纳米羟基磷灰石/细菌纤维素复合组织工程支架的细胞毒性和生物相容性[J].中国组织工程研究,2014,(47):7615-7620,6.基金项目
江苏省“科教兴卫”医学重点人才项目(H201129) （H201129）
