中国组织工程研究Issue(50)：8037-8041,5.DOI:10.3969/j.issn.2095-4344
Ad-hBMP-2与Ad-hTGF-β1基因共转染骨髓间充质干细胞后的体外成骨诱导
In vitro osteogenic induction of bone marrow mesenchymal stem cells after co-transfection with Ad-hBMP-2 and Ad-hTGF-β1
陈剑平 1邱俊钦 1曾昭勋 1陈宗雄 1尹承慧1
作者信息
- 1. 解放军南京军区福州总医院骨一科，福建省福州市 350025
- 折叠
摘要
Abstract
BACKGROUND:Advances in recombinant DNA technology provide a huge impetus to the development of bone tissue engineering research, which used for tissue-engineered bone construction can significantly improve the quality and efficiency of bone repair. <br> OBJECTIVE:To evaluate the in vitro osteogenic induction of Ad-hBMP-2 and Ad-hTGF-β1 co-transfected bone marrow mesenchymal stem cels and to elucidate their relationship in bone formation. <br> METHODS:Bone marrow mesenchymal stem cels from Sprague-Dawley rats were infected by high-titer recombinant adenoviruses carrying transforming growth factor-β1 and bone morphogenetic protein-2 genes, and then transfected cels were induced by exogenous genes detected with RT-PCR, ELISA, MTT and alkaline phosphatase test. <br> RESULTS AND CONCLUSION:Adenovirus-infected bone marrow mesenchymal stem cels could express exogenous genes detected by RT-PCR. MTT test and alkaline phosphatase test showed the proliferation ability of co-transfected bone marrow mesenchymal stem cels was the strongest and the alkaline phosphatase activity was the highest. It has been observed that bone marrow mesenchymal stem cels co-transfected with Ad-hBMP-2 and Ad-hTGF-β1 can be induced to form bone tissue cooperatively and synergisticaly.关键词
干细胞/骨髓干细胞/转化生长因子β1/骨形态发生蛋白2/腺病毒载体Key words
bone marrow/mesenchymal stem cels/transforming growth factor beta1/bone /morphogenetic proteins分类
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陈剑平,邱俊钦,曾昭勋,陈宗雄,尹承慧..Ad-hBMP-2与Ad-hTGF-β1基因共转染骨髓间充质干细胞后的体外成骨诱导[J].中国组织工程研究,2014,(50):8037-8041,5.
