中国组织工程研究Issue(50)：8088-8092,5.DOI:10.3969/j.issn.2095-4344
右美托咪定对脐血间充质干细胞向神经细胞分化的影响
Effect of dexmedetomidine on neuronal differentiation of umbilical cord blood mesenchymal stem cells
樊志刚 1乔蕾1
作者信息
- 1. 河南医学高等专科学校，河南省郑州市 451191
- 折叠
摘要
Abstract
BACKGROUND:Increasing evidence has shown that dexmedetomidine has a significant neuroprotection against ischemia-reperfusion injury, but its mechanism of action on the nervous system and its pathways to affect nerve cel function are rarely reported. <br> OBJECTIVE: To study the effect of dexmedetomidine on proliferation and neuronal differentiation of umbilical cord blood mesenchymal stem cels. <br> METHODS:Umbilical cord blood mesenchymal stem cels at passage 3 were divided into control, low-concentration (1 μg/L) and high-concentration (10 μg/L) dexmedetomidine groups. MTT was used to detect the viability of umbilical cord blood mesenchymal stem cels; western blot assay was used to determine the ERK1/2 phosphorylation in the cels; immunofluorescence staining was used to detect the percentage of NeuN/DAPI, GFAP/DAPI and Nestin/DAPI positive cels. Cel differentiation and proliferation abilities were observed. <br> RESULTS AND CONCLUSION:Cel viability and EKR1/2 phosphorylation level were significantly increased in the low-concentration group compared to the control group (P < 0.05); while these two indicators in the high-concentration group were significantly lower than those in the other two groups (P < 0.05). The number of NeuN and GFAP positive cels was increased significantly in the two dexmedetomidine groups compared with the control group (P < 0.05), while the number of Nestin positive cels was significantly decreased in the two dexmedetomidine groups (P < 0.05). These findings indicate that low-concentration dexmedetomidine can induce the proliferation of umbilical cord blood mesenchymal stem cels that is however inhibited by the high-concentration dexmedetomidine, which can be realized by regulating EKR1/2 phosphorylation level. In addition, low- and high-concentration dexmedetomidine can both induce the neuronal differentiation of umbilical cord blood mesenchymal stem cels.关键词
干细胞/脐带脐血干细胞/右美托咪定/增殖:分化Key words
fetal blood/mesenchymal stem cels/dexmedetomidine/cel proliferation分类
医药卫生引用本文复制引用
樊志刚,乔蕾..右美托咪定对脐血间充质干细胞向神经细胞分化的影响[J].中国组织工程研究,2014,(50):8088-8092,5.
