中国组织工程研究Issue(51)：8337-8345,9.DOI:10.3969/j.issn.2095-4344
差异蛋白基因生物信息学在冠心病血瘀证遗传相关验证中的应用
Bioinformatics analysis of differential genes correlated with blood stasis syndrome of coronary heart disease
摘要
Abstract
BACKGROUND:Previous findings on clinical epidemiology and molecular epidemiology have shown that blood stasis syndrome of coronary heart disease has a certain familial aggregation tendency, and its related differential genes are closely associated with inflammation, plaque formation and vascular endothelial damage. <br> OBJECTIVE: To analysis the bioinformatics of differential genes correlated with blood stasis syndrome of coronary heart disease. <br> METHODS: Based on the epidemiological survey, differential genes related to blood stasis syndrome of coronary heart disease were identified using gene chip technology. The latest versions of bioinformatics tools including DAVID, GO were combined to assign a precise function to differential genes correlated with coronary heart disease; KEGG, BIOCARTA and BBIOD were used to analyze the signaling pathway; VISANT and DIP to retrieve precise protein-protein interactions. <br> RESULTS AND CONCLUSION:Differential genes were mainly involved in 43 biological processes, 3 celular components, and 6 molecular functions. These proteins mainly participated in 10 signal transduction pathways, and interacted in many ways. There were 189 nods and 5 path mappings, and overal 29 nod functions were observed. The functions, signal transduction pathways and interactions of differential genes related to blood stasis syndrome of coronary heart disease are mainly reflected in inflammatory response, immune response, cytokine chemotaxis and cel proliferation. Genome bioinformatics technology can make predictions of TCM syndromes from a “holistic view”, and this technology further combined with molecular biology validation is a very good way in the modernization of Chinese medicine.关键词
组织构建/组织工程/冠心病/血瘀证/遗传/基因组学/生物信息学/国家自然科学基金Key words
Subject headings:heart diseases/atherosclerosis/genomics分类
医药卫生引用本文复制引用
郑景辉,刘吉勇,袁肇凯,张敏州,李杰,王丽萍,宁桂兰,孙贵香,简维雄,黄献平..差异蛋白基因生物信息学在冠心病血瘀证遗传相关验证中的应用[J].中国组织工程研究,2014,(51):8337-8345,9.基金项目
国家自然科学基金青年项目(81102595)；中国博士后科学基金特别资助项目(2014T70800)；广西自然科学基金课题(2012GXNSFAA053113)；广西壮族自治区卫生厅中医药科技专项课题(GZPT1219)；广西壮族自治区卫生厅自筹经费科研课题(Z2012181)Funding:the National Natural Science Foundation of China for the Youth, No.81102595 （81102595）
China Postdoctoral Foundation, No.2014T70800 （）
the Natural Science Foundation of Guangxi Zhuang Autonomous Region, No.2012GXNSFAA053113 （）
the Special Project of Traditional Chinese Medicine of the Health Department of Guangxi Zhuang Autonomous Region, No. GZPT1219 （）
the Self-Financing Scientific Research Project of the Health Department of Guangxi Zhuang Autonomous Region, No. Z2012181 （）
