中国组织工程研究Issue(52)：8387-8392,6.DOI:10.3969/j.issn.2095-4344
钛基表面纳米银复合涂层体外抗菌活性及生物安全性评价
In vitro antibacterial activity and biological safety of nano-silver composite coating on the surface of titanium base
摘要
Abstract
BACKGROUND:Hydroxyapatite is the main component of bone with the capacity of osteogenic induction. Nano-silver has broad-spectrum and efficient antibacterial activity, while hydroxyapatite/nano-silver composite coating on the surface of titanium base has either biological activity or antimicrobial properties. <br> OBJECTIVE:To study the antimicrobial properties of hydroxyapatite/nano-silver composite coating on the surface of titanium base and to evaluate the biocompatibility and security of hydroxyapatite/nano-silver composite coating on the surface of titanium base. <br> METHODS: Silver solution of 0, 0.5, 1.0 mmol/L was selected to prepare hydroxyapatite/nano-silver composite coating samples deposited on the surface of titanium base. Then,Staphylococcus aureus was cultured in the sample leaching solution for thein vitro antibacterial qualitative analysis. In accordance with national and international standardization for biocompatibility testing, healthy adult Kunming mice and New Zealand rabbits were subjected to pyrogen test, hemolysis test, acute toxicity test, skin irritation test for comprehensive evaluation of the material biocompatibility and safety. <br> RESULTS AND CONCLUSION:The hydroxyapatite/nano-silver composite coating on the surface of titanium base exhibited obvious antibacterial activityin vitro againstStaphylococcus aureus, in a silver dose-dependent manner. There was no pyrogen response, hemolytic reaction, acute toxic reaction and skin stimulus response in the animal experiments. There was also no difference in the biocompatibility of hydroxyapatite materials containing silver or not. These findings indicate that the hydroxyapatite/nano-silver composite coating on the surface of titanium base has good antibacterial activity for Staphylococcus aureusin vitro as wel as excelent biocompatibility.关键词
生物材料/纳米生物材料/纳米银/医用钛/组织相容性/羟基磷灰石/骨科植入物/脉冲电泳涂层/抗菌活性/金黄色葡萄球菌/生物安全性/新疆维吾尔自治区自然科学基金Key words
Subject headings:hydroxyapatites/silver/Staphylococcus aureus/titanium分类
医药卫生引用本文复制引用
鲜爱明,张晓岗,曹力,徐恩洁,彭理斌,胡洋..钛基表面纳米银复合涂层体外抗菌活性及生物安全性评价[J].中国组织工程研究,2014,(52):8387-8392,6.基金项目
Funding：the Natural Science Foundation of Xinjiang Uygur Autonomous Region, No.2013211A075 （）
