中国组织工程研究Issue(52)：8433-8437,5.DOI:10.3969/j.issn.2095-4344
聚羟基脂肪酸酯和聚酰胺-胺树状高分子构筑的经皮给药系统
Transdermal drug delivery system constructed by polyhydroxyalkanoates and PAMAM dendrimers
摘要
Abstract
BACKGROUND:Polyhydroxyalkanoates (PHA) can be used as anin vitro drug delivery carrier for its characteristics of viscosity and ability of drug mixture and dispersion. PAMAM dendrimers have unique physical and chemical properties as a drug carrier. <br> OBJECTIVE:To mix PHA with PAMAM to build a transdermal drug delivery system for indissolvable drugs. <br> METHODS:Pseudomonas putida KT2442 was cultured in a culture medium containing NH4Cl at 30℃ for 72 hours, then 5 g/L octane acid was added in the culture system and cultured for 24 hours, and PHA compound was extracted with chloroform. PHA compound matrix (10 mg) and PHA compound matrix containing PAMAM and phenethylamine dendrimers (10 mg) were respectively mixed with 5 mg of sulfonamide derivative Tamsulosin to construct a transdermal delivery system. Meanwhile, anin vitro permeation device was established to study the promoting effect of compound matrices on drug penetration. X-ray diffraction analysis was used to analyze the atomic spatial distribution of Tamsulosin, PHA, PHA compound matrix-Tamsulosin, and PHA compound matrix containing PAMAM dendrimers-Tamsulosin. <br> RESULTS AND CONCLUSION: PHA and mixture of PHA and PAMAM dendrimers could promote the transdermal effect of indissolvable drugs as a transdermal drug matrix. In the same condition, the mixture of PHA and PAMAM dendrimers could satisfy the clinical blood drug concentration as a transdermal drug matrix. This mixture can make drug model crystalized wel in a highly ordered manner.关键词
生物材料/缓释材料/聚羟基脂肪酸酯/聚酰胺-胺树状高分子/经皮给药系统/TamsulosinKey words
Subject headings：polyhydroxyalkanoates/administration, cutaneous/osmosis分类
医药卫生引用本文复制引用
缪伟伟,王志雄,张俊利,大野優子..聚羟基脂肪酸酯和聚酰胺-胺树状高分子构筑的经皮给药系统[J].中国组织工程研究,2014,(52):8433-8437,5.基金项目
Funding：Shanghai Education Commission Scientific Research Innovation Projects (Natural Science Class), No.11YZ293上海市教育委员会科研创新项目(自然科学类)(11YZ293)，项目名称用生物合成生分解材料mcl-PHA构筑经皮给药系统 （Natural Science Class）
