中国组织工程研究Issue(52)：8509-8514,6.DOI:10.3969/j.issn.2095-4344
次氯酸钠溶液对不同时期粪肠球菌生物膜的药物作用
Antibacterial effect of sodium hypochlorite on in vitro biofilm formation of Enterococcus faecalis in different phases
摘要
Abstract
BACKGROUND:Studies have reportedEnterococcus faecalis can exist in environment which is lack of nutrients and antimicrobials to cause re-infection of the root canal. Sodium hypochlorite solution is shown to have strong scavenging action as a root canal flushing fluid to eliminate residualEnterococcus faecalis. <br> OBJECTIVE:To explore the antibacterial effect of sodium hypochlorite onin vitro biofilm formation of Enterococcus faecalis in different phases. <br> METHODS:Biofilm models of Enterococcus faecalis were established at exponential phase, stationary phase and starvation phase, and then the biofilms were exposed to various concentrations of sodium hypochlorite solutions (1.0%, 2.5%, 5.25%). The contact time was 30 seconds, 5 minutes and 10 minutes. The direct optical observation ofEnterococcus faecalis was conducted under confocal laser scanning microscope. <br> RESULTS AND CONCLUSION:In the same concentration of sodium hypochlorite solutions, the biofilms of starved cels were more resistant to sodium hypochlorite than those formed in the exponential phase and stationary phase (P < 0.05). When the concentration of sodium hypochlorite was 1%, the reduction of viable cels showed significant difference between the exponential phase and stationary phase (P < 0.05). When the concentration of sodium hypochlorite was 2.5% and 5.25%, the reduction of viable cels in the exponential phase and stationary phase had no statistical difference (P > 0.05). For the biofilms at the same phase, 5.25% sodium hypochlorite reduced more viable cels than 2.5% and 1% sodium hypochlorite (P < 0.05), and 2.5% sodium hypochlorite also could reduce more viable cels than 1% sodium hypochlorite, but it only exhibited a significant difference when the contact time was 30 seconds (P < 0.05). These results show that under the same concentration and same contact time, theEnterococcus faecalis biofilms at the starvation phase are more resistant to sodium hypochlorite than those at the exponential phase and stationary phase. Under the same contact time, 5.25% sodium hypochlorite exhibits the best antibacterial effect on theEnterococcus faecalis biofilms at the starvation phase.关键词
生物材料/口腔生物材料/次氯酸钠溶液/粪肠球菌/生物膜/饥饿期/国家自然科学基金Key words
Subject headings：sodium hypochlorite/Enterococcus faecalis/biofilms分类
医药卫生引用本文复制引用
王珊珊,董明,王丽娜,牛卫东..次氯酸钠溶液对不同时期粪肠球菌生物膜的药物作用[J].中国组织工程研究,2014,(52):8509-8514,6.基金项目
国家自然科学基金项目(81171538)Funding:the National Natural Science Foundation of China. No.81171538 （81171538）
