中国组织工程研究Issue(1)：101-107,7.DOI:10.3969/j.issn.2095-4344.2015.01.018
芍药苷对骨髓间充质干细胞增殖的影响
Effects of paeoniflorin on the proliferation of bone marrow mesenchymal stem cells
摘要
Abstract
BACKGROUND:Studies have shown that paeoniflorin functions as replenishing blood and treatment of autoimmune diseases, and bone marrow mesenchymal stem cels also play an important role in the body’s blood and immune function. However, paeoniflorin effects on bone marrow mesenchymal stem cel proliferation and cytokine secretion and expression are rarely reported. OBJECTIVE:To investigate the effect of paeoniflorin on proliferation of bone marrow mesenchymal stem cels and the expression of interleukin-6. METHODS:Human bone marrow mesenchymal stem cels were separated and culturedin vitro by density gradient centrifugation combined with attachment method. The biological characteristics of bone marrow mesenchymal stem cels were identified by flow cytometry and osteogenic/adipogenic induction. The proliferation of bone marrow mesenchymal stem cels under different concentrations of paeoniflorin was detected by MTT method. The mRNA expression and secretion of interleukin-6 in the supernatant of bone marrow mesenchymal stem cels were detected by RT-PCR and ELISA, respectively. RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cels were isolated successfuly and had osteogenic and adipogenic differentiation potential. Compared with the controlgroup, 2 μmol/L and 10 μmol/L paeoniflorin significantly promoted the proliferation of bone marrow mesenchymal stem cels. 10 μmol/L paeoniflorin could significantly decrease the proportion of bone marrow mesenchymal stem cels in G0/G1 phase and increase this proportion in S phase. Compared with the control group, the experimental group could significantly increase the secretion and mRNA expression of interleukin-6 (P < 0.01). It is concluded that paeoniflorin at certain concentrations can obviously promote the proliferation of bone marrow mesenchymal stem cels, and increase the expression and secretion of interleukin-6.关键词
干细胞/骨髓干细胞/芍药苷/骨髓间充质干细胞/细胞增殖/白细胞介素6Key words
Bone Marrow/Mesenchymal Stem Cells/Cell Proliferation/Interleukin-6分类
医药卫生引用本文复制引用
晁二涛,白海,王存邦,葸瑞,欧剑锋,赵强..芍药苷对骨髓间充质干细胞增殖的影响[J].中国组织工程研究,2015,(1):101-107,7.基金项目
甘肃省科技重大专项(1102FKDA005)@@@@ the Major Science and Technology Project of Gansu Province, No.1102FKDA005 （1102FKDA005）
