中国组织工程研究Issue(2)：170-176,7.DOI:10.3969/j.issn.2095-4344.2015.02.002
去卵巢大鼠不同时期骨量、骨转换指标、雌激素水平的变化规律及相关性
Change rules and correlation between bone mass, bone turnover markers and estrogen levels in different periods of ovariectomized rats
摘要
Abstract
BACKGROUND:There are so many studies about ovariectomized rats at present, but the research on the change rules of bone mass, bone turnover markers, estrogen levels and their correlation in different periods of ovariectomized rats is rarely reported. OBJECTIVE:To analyze the change rules of bone mass, bone turnover markers, estrogen level and their correlation in different periods of ovariectomized rats. METHODS: Thirty-four 3-month-old female Sprague Dawley rats were randomly divided into three groups: baseline group, ovariectomized group and sham operated group. At the beginning of the experiment, the rats in the baseline group were sacrificed, then rats in the ovariectomized group and sham operated group were executed at 4, 8, 12 weeks postoperative respectively. The bone mineral density, bone mass content, area of different zones of the L1-3 lumbar vertebrae and femurs were detected by dual-energy X-ray absorption method, and meanwhile the serum levels of type I procolagen amino-terminal pro-peptide, I colagen carboxy-terminal peptide and estrogen were determined by ELISA. At last, we analyzed the correlation between body mass, bone mineral densityin vitro, type I procolagen amino-terminal pro-peptide, I colagen carboxy-terminal peptide and estrogen levels and the age of ovariectomized rats. RESULTS AND CONCLUSION: (1) The bone mineral density and bone mass content of the lumbar vertebral and femurs in the ovariectomized group were significantly lower than those in the sham operated group and baseline group at the 4th week after operation (P < 0.05). The bone mineral density and bone mass content in the ovariectomized group were ameliorated obviously at the 8th and 12th weeks compared with those at the 4th week after operation (P < 0.05). The bone mass loss was highest in the L1 and intertrochanteric regions. (2) Serum levels of type I procolagen amino-terminal pro-peptide and I colagen carboxy-terminal peptide in the ovariectomized group were significantly higher than those in the baseline group and sham operated group at the 4th week after operation, but there was no difference at the 8th and 12th weeks. (3) The serum estrogen level in the ovariectomized group was prominently lower than that in the sham operated group and baseline group at the 8th and 12th weeks after operation (P < 0.01 at the 8th week,P < 0.05 at the 12th week). (4) The age was positively correlated with body mass and bone mineral density of the lumbar vertebrae and femursin vitro, while the serum levels of type I procolagen amino-terminal pro-peptide and I colagen carboxy-terminal peptide were negatively correlated with the bone mineral density of the lumbar vertebrae and femurs in vitro (P < 0.01). These results suggested that the bone mass of the lumbar vertebrae and femurs in ovariectomized rats was decreased rapidly firstly, and then rose slowly with time; the bone mass in the L1 and intertrochanteric regions lost seriously; the bone turnover markers showed a significant increase at the beginning of ovariectomy and reduced gradualy to normal condition, while the estrogen level was increased at the first month after ovariectomy and then decreased rapidly. In addition, the body mass, bone turnover markers and estrogen level were associated with the change of bone mass.关键词
组织构建/骨组织工程/去卵巢大鼠/不同时期/骨量/Ⅰ型前胶原氨基端原肽/Ⅰ型胶原羧基端肽/雌激素/变化规律/相关性/绝经后骨质疏松症/广东省自然科学基金Key words
Osteoporosis, Postmenopausal/Estrogens/Ovariectomy分类
医药卫生引用本文复制引用
沈耿杨,张顺聪,姚珍松,任辉,江晓兵,梁德,杨志东,唐晶晶,崔健超,林顺鑫,庄洪..去卵巢大鼠不同时期骨量、骨转换指标、雌激素水平的变化规律及相关性[J].中国组织工程研究,2015,(2):170-176,7.基金项目
广东省教育厅学科建设专项基金(育苗工程)(2013LYM-0012)；广东省科技厅资助项目(2012B031800208)；广东省自然科学基金项目(S2013010015870)；广州中医药大学优秀青年学者科研基金项目(KAB110133K04) （育苗工程）
