中国组织工程研究Issue(10)：1570-1574,5.DOI:10.3969/j.issn.2095-4344.2015.10.017
干细胞与膀胱脱细胞基质构建组织工程管状移植物修复长段输尿管缺损
Tissue-engineered tubular grafts constructed with stem cells and bladder acellular matrix in long-segmental ureteral reconstruction
摘要
Abstract
BACKGROUND:The main way for long-segmental ureteral reconstruction may cause a lot of traumas and complications. Therefore, to seek a new repair method is urgent. OBJECTIVE:To investigate the feasibility of a tissue-engineered tubular graft for ureteral reconstruction. METHODS:Bone marrow mesenchymal stem cels and smooth muscle cels of rabbits were seeded into the two surfaces of bladder acelular matrix and cultivated for 7 days. Then the graft was used to prepare a 4-cm long tissue-engineered tubular graft, which was regarded as experimental group. Smooth muscle cels seeded onto the bladder acelular matrix was used to construct the tissue-engineered tubular graft as control group. Twenty-five New Zealand rabbits were randomly divided into experimental group (n=20) and control group (n=5), and two kinds of tubular grafts covered with omentum were implanted into the two groups, respectively, for repair of ureteral defects. Hematoxylin-eosin staining and immunohistochemical detection were performed at 2, 4, 8 weeks after implantation. RESULTS AND CONCLUSION:In the experimental group, hematoxylin-eosin staining showed epithelial coverage and muscle fibers on the lumen of tissue-engineered tubular grafts at 8 weeks after implantation; immunohistochemistry showed that anti-AE1/AE3 antibody and anti-uroplakinⅢa antibody were positive, confirming that there were mature epithelial cels on the lumen of tissue-engineered tubular grafts. In the control group, five rabbits were dead within 2 weeks after removal of ureteral scaffold, and autopsy showed scar formation inside the graft and severe hydronephrosis. These results demonstrate that it is feasible to construct the tissue-engineered tubular graft using bone marrow mesenchymal stem cels and smooth muscle cels into the bladder acelular matrix for ureteral reconstruction. Bone marrow mesenchymal stem cels can potentialy promote urothelial regeneration.关键词
干细胞/移植/膀胱脱细胞基质/骨髓间充质干细胞/平滑肌细胞/输尿管缺损/尿路上皮再生/组织工程/国家自然科学基金Key words
Bone Marrow/Mesenchymal Stem Cels/Myocytes, Smooth Muscle/Ureteral Diseases分类
医药卫生引用本文复制引用
廖文彪,杨嗣星,熊云鹤,宋超,孟令超..干细胞与膀胱脱细胞基质构建组织工程管状移植物修复长段输尿管缺损[J].中国组织工程研究,2015,(10):1570-1574,5.基金项目
国家自然科学基金项目(31400835)Funding:the National Natural Science Foundation of China, No.31400835 （31400835）
