中国组织工程研究Issue(10)：1585-1591,7.DOI:10.3969/j.issn.2095-4344.2015.10.020
脂肪与髓核来源间充质干细胞在转化生长因子β1诱导下向类髓核细胞分化
Mesenchymal stem cells derived from adipose and nucleus pulposus tissue differentiate towards nucleus pulposus-like cells induced by transforming growth factor-beta 1
摘要
Abstract
BACKGROUND:A large number of studies have shown that adult stem cels derived from multiple tissues are available to differentiate towards nucleus pulposus-like celsin vitro. It is unclear whether mesenchymal stem cels derived from nucleus pulposus tissues have the ability to differentiate towards nucleus pulposus-like phenotypes induced by transforming growth factor-beta 1. Up to now, there are few reports on the difference between the differentiation ability of mesenchymal stem cels derived from nucleus pulposus tissues and adipose-derived mesenchymal stem cels. OBJECTIVE:To compare the ability of mesenchymal stem cels derived from nucleus pulposus tissues and adipose-derived mesenchymal stem cels differentiating into nucleus pulposus-like cels under induction of transforming growth factor-beta 1. METHODS:The groin fat tissue and the coccygeal spine of rats were taken respectively to isolate and culture mesenchymal stem cels derived from nucleus pulposus tissues and adipose-derived mesenchymal stem cels by mechanical enzyme digestion method. Flow cytometry was employed to detect the expression of CD105, CD90, CD29, CD45, CD44, CD34, and CD24 of both two kinds of stem cels. Mesenchymal stem cels derived from nucleus pulposus tissues and adipose-derived mesenchymal stem cels were divided into complete induction group (complete induction medium with transforming growth factor-beta 1), incomplete induction group (complete induction medium without transforming growth factor-beta 1) and control group(DMEM/F12 containing 10% fetal bovine serum and 100 mg/L penicilin/streptomycin), respectively. After 14 days of culture, real-time PCR was used to detect the expression of colagen type II, Aggrecan and SOX-9 in each group. RESULTS AND CONCLUSION:CD105, CD90, CD29 expressed positively and CD45, CD44, CD34, CD24 negatively in both two kinds of stem cels. After 14 days of induced differentiation, the expressions of colagen type II, Aggrecan and SOX-9 in the two kinds of cels were significantly higher in the complete induction groups than in the control groups (P < 0.05). Under the induction of transforming growth factor-beta 1, the expression of colagen type II, Aggrecan and SOX-9 in mesenchymal stem cels derived from nucleus pulposus tissues was significantly higher than that in adipose-derived mesenchymal stem cels (P < 0.05). These findings suggest that both two kinds of mesenchymal stem cels have the ability to differentiate towards nucleus pulposus-like cels induced by transforming growth factor-beta, and mesenchymal stem cels derived from nucleus pulposus tissues may be more suitable as seed cels for nucleus pulposus tissue engineering research.关键词
干细胞/脂肪干细胞/髓核间充质干细胞/椎间盘/转化生长因子β1/诱导分化Key words
Adipose Tissue/Intervertebral Disk/Mesenchymal Stem Cels/Transforming Growth Factor beta1分类
医药卫生引用本文复制引用
薛晨晖,马迅,关晓明,张辉,张丽..脂肪与髓核来源间充质干细胞在转化生长因子β1诱导下向类髓核细胞分化[J].中国组织工程研究,2015,(10):1585-1591,7.基金项目
山西医科大学科技创新基金(01201114)Funding:the Scientific Innovation Foundation of Shanxi Medical University, No.01201114 （01201114）
