中国组织工程研究Issue(11)：1668-1672,5.DOI:10.3969/j.issn.2095-4344.2015.11.006
低强度脉冲超声波联合骨形态发生蛋白2促进人牙周膜细胞的成骨分化
Osteogenic effect of low-intensity pulsed ultrasound combined with bone morphogenetic protein 2 on human periodontal ligament cells
摘要
Abstract
BACKGROUND:Both of low-intensity pulsed ultrasound (LIPUS) and bone morphogenetic protein-2 (BMP-2) can promote the osteogenic differentiation of human periodontal ligament cels, but there is no report on their combination application. OBJECTIVE:To evaluate the biological effect of LIPUS combined with BMP-2 on osteogenic differentiation of human periodontal ligament cels. METHODS:Human periodontal ligament cels were isolated, cultured, passaged and identified. Passage 4 cels were seeded onto 6-wel plates and divided into four groups: control group, LIPUS group, BMP-2 group, LIPUS+BMP-2 group. Alkaline phosphatase activity was detected by alkaline phosphatase activity measurement kit, and the expression of type I colagen and osteopontin was detected by RT-PCR. RESULTS AND CONCLUSION: After LIPUS, BMP-2, and their combination treatment, the activity of alkaline phosphatase was significantly higher than that in the control group (P < 0.05), and the activity of alkaline phosphatase was highest in the LIPUS+BMP-2 group (P < 0.05). RT-PCR results showed that both of LIPUS and BMP-2 could augment the mRNA expression of type I colagen and osteopontin (P < 0.01), but it was more <br> distinct when they were used together (P < 0.001). In a conclusion, the combined utilization of LIPUS and BMP-2 shows stronger ability in promoting the osteogenic differentiation of human periodontal ligament cels.关键词
组织构建/骨组织工程/低强度脉冲超声/骨形态发生蛋白2/人牙周膜细胞/成骨分化/国家自然科学基金Key words
Sonication/Bone Morphogenetic Proteins/Periodontal Ligament分类
医药卫生引用本文复制引用
刘俊,胡波,蒋欣益,孙吉成,邓锋,宋锦璘..低强度脉冲超声波联合骨形态发生蛋白2促进人牙周膜细胞的成骨分化[J].中国组织工程研究,2015,(11):1668-1672,5.基金项目
国家自然科学基金(30870754)，课题名称基于牙周组织工程技术的低强度脉冲超声波促进牙周病组织修复作用研究@@@@the National Natural Science Foundation of China, No. 30870754 （30870754）
