中国组织工程研究Issue(11)：1688-1693,6.DOI:10.3969/j.issn.2095-4344.2015.11.010
血小板衍化生长因子DD刺激大鼠肾成纤维细胞增殖及向肌成纤维细胞的转化
Platelet-derived growth factor-DD induces the proliferation and differentiation of rat renal fibroblasts into myofibroblasts
摘要
Abstract
BACKGROUND:Platelet-derived growth factors can induce proliferation, migration, transformation and extracelular matrix expression of glomerular mesangial cels and renal interstitial cels. OBJECTIVE:To investigate the effect of activation of platelet-derived growth factor-DD/βR signaling pathway on the proliferation and α-smooth muscle actin expression in rat renal fibroblasts. METHODS: Normal rat kidney interstitial fibroblasts (NRK-49F) cultured in vitro were divided into the folowing groups by platelet-derived growth factor-DD concentrations: control, 1 μg/L, 10 μg/L, 50 μg/L, 100 μg/L. And NRK-49Fs were then divided into four groups according to the stimulation time of 50 μg/L platelet-derived growth factor-DD:control, 12 hours, 24 hours, 48 hours. The cel viability of the NRK-49Fs was assessed by Cel Counting Kit-8 after platelet-derived growth factor-DD administration. The mRNA and protein expression levels of <br> platelet-derived growth factor-βR and α-smooth muscle actin in al groups stimulated by different concentrations of platelet-derived growth factor-DD were determined by RT-PCR and western blot, respectively. RESULTS AND CONCLUSION:Platelet-derived growth factor-DD could facilitate the proliferation rates of the NRK-49Fs at a dose- and time-dependent manner as compared with the control groups. Platelet-derived growth factor-DD could stimulate the mRNA and protein expressions of platelet-derived growth factor-βR and α-smooth muscle actin in a dose-dependent manner. This suggest that the activation of platelet-derived growth facto-DD/βR signaling pathway can obviously promote the proliferation of NRK-49Fs as wel as tranformation into myofibroblasts.关键词
组织构建/成纤维细胞/肾间质纤维化/大鼠/肾成纤维细胞/血小板衍化生长因子DD/细胞增殖/肌成纤维细胞/α-平滑肌肌动蛋白Key words
Kidney/Fibrosis/Fibroblasts/Phenotype/Receptors, Platelet-Derived Growth Factor分类
医药卫生引用本文复制引用
赵佳,孙建平,高延霞,唐妮娜,牛蒙,崔萌,韩晓庆,隋爱华..血小板衍化生长因子DD刺激大鼠肾成纤维细胞增殖及向肌成纤维细胞的转化[J].中国组织工程研究,2015,(11):1688-1693,6.基金项目
青岛市科技计划基础研究项目[11-2-4-2-(12)-jch]@@@@the Basic Research Project of Qingdao Science and Technology Plan, No. 11-2-4-2-(12)-jch （12）
