中国组织工程研究Issue(12)：1858-1863,6.DOI:10.3969/j.issn.2095-4344.2015.12.011
加入壳聚糖改善丝素蛋白结晶性：力学稳定强度更好的三维支架材料
Chitosan improves the crystallization of silk fibroin：a three-dimensional scaffold material with better mechanical stability
摘要
Abstract
BACKGROUND:Silk fibroin as natural biological macromolecules has good biocompatibility, but it is difficult to make the three-dimensional scaffold with uniform structure because of its higher crystalization performance and bigger brittleness. OBJECTIVE:To improve the crystalization of silk fibroin through the addition of chitosan, and to get three-dimensional tissue engineering scaffolds with better mechanical strength. METHODS: CaCl2/CH3CH2OH/H2 RESULTS AND CONCLUSION:The introduction of chitosan could improve the properties of scaffolds. The porosity of the composite scaffold with lower porosity was more uniform and orderly with higher content of O ternary solution was used to dissolve silkworm cocoon to extract silk fibroin and form solution. Silk fibroin solution and chitosan solution were mixed according to different mixing ratios of 2:1, 1:1, 1:2, respectively, and then porous silk protein/chitosan scaffolds were prepared by freeze-drying method and treated by methanol. Scaffold morphology was observed by scanning electron microscopy, the chemical structure and crystaline state of the scaffolds were characterized through infrared spectrum and X-ray diffraction test, respectively. Also, the porosity and water uptake were tested and periodic cycle compression mechanical properties under the water environment were determined. chitosan. When the mixture rate of chitosan and silk fibroin was 1:2, the water uptake rate was the highest in the composite scaffolds, and also higher than that of the silk fibroin scaffold but lower than that of the chitosan scaffold. With the increase of silk fibroin, the composite scaffolds had better elasticity and stronger ability to maintain the shape.关键词
生物材料/材料相容性/丝素蛋白/壳聚糖/复合支架/性能研究/国家自然科学基金Key words
Materials Testing/Chitosan/Silk分类
医药卫生引用本文复制引用
张霞只,司徒方民,彭鹏,焦延鹏..加入壳聚糖改善丝素蛋白结晶性：力学稳定强度更好的三维支架材料[J].中国组织工程研究,2015,(12):1858-1863,6.基金项目
the National Natural Science Foundation of China, No.50903039,81101151 （）
the Research Fund for the Excelent Doctorial Dissertation of Guangdong Province, No. sybzzxm201034基金资助国家自然科学基金青年、面上项目(50903039、81101151)；广东省优秀博士论文自助项目(sybzzxm201034) （50903039、81101151）
