中国组织工程研究Issue(15)：2297-2304,8.DOI:10.3969/j.issn.2095-4344.2015.15.001
抵抗素刺激软骨细胞趋化因子CCL3及CCL4基因表达及机制
Resisin stimulates the expression of CCL3 and CCL4 in chondrocytes
摘要
Abstract
BACKGROUND:Previous studies have indicated that resistin stimulates a large set of chemokines in chondrocytes that are known to be important in inflammatory joint lesions. <br> OBJECTIVE:To further investigate the mechanism of co-regulation roles of transcription and post-transcription in the up-regulation of two chemokine genes CCL3 and CCL4 in chondrocytes in response to resistin. <br> METHODS:Human chondrocytes, T/C-28a2 and ATDC5 cels were cultured. The function of resistin on the chemokine genes, and the expression of C/EBPβ, nuclear factor-κB isoforms and chondrogenic specific miRNAs were tested by qPCR. The co-regulation of C/EBPβ and nuclear factor-κB was investigated by nuclear factor-κB inhibitor (IKK-NBD) and C/EBPβ inhibitor (SB303580) treatments, and subcelular localization was detected with or without resistin stimulation. <br> RESULTS AND CONCLUSION:Resistin could increase the expression of chemokine genes independently. Chondrocytes reacted in a non-restrictedly cel-specific manner to resistin; C/EBPβ inhibitor, nuclear factor-κB and some chondrogenic specific miRNAs in a combinatorial manner regulated chemokine gene expression. The activity of C/EBPβ was augmented by a transient increase in activity of nuclear factor-κB, and both transcription factors acted independently on the chemokine genes, CCL3 and CCL4.关键词
组织构建/软骨组织工程/抵抗素/软骨细胞/趋化因子/CCL3/CCL4/转录调控/C/EBPβ/核因子κB/转录后调控/miRNAs/国家自然科学基金Key words
Subject headings:Chondrocytes/Chemotactic Factors/NF-kappa B分类
医药卫生引用本文复制引用
张紫机,廖威明,张志奇,康焱,杨子波,侯昌禾,黄广鑫,陈蔚深,盛璞义,何爱珊,傅明..抵抗素刺激软骨细胞趋化因子CCL3及CCL4基因表达及机制[J].中国组织工程研究,2015,(15):2297-2304,8.基金项目
国家自然科学基金资助项目(81171709，81301558，81371941，81201388)；教育部高等学校博士点基金新教师类资助项目(20130171120074)；广东省自然科学基金博士启动项目(S2013040016269)Funding:the National Natural Science Foundation of China, No.81171709,81301558,81371941,81201388 （81171709，81301558，81371941，81201388）
the National Research Foundation for the Doctoral Program of Higher Education, No.20130171120074 （）
the Natural Science Foundation of Guangdong Province, No. S2013040016269 （）
