中国组织工程研究Issue(18)：2800-2806,7.DOI:10.3969/j.issn.2095-4344.2015.18.003
构建股骨骨折合并脑损伤模型大鼠低氧诱导因子1α和核心结合因子α1的表达
Expression of hypoxia-inducible factor 1 alpha and core binding factor alpha 1 in rat models of femoral fracture combined with cerebral trauma
摘要
Abstract
BACKGROUND:The low oxygen environment after femoral fracture and cerebral trauma wil induce series of related cytokines expression, including hypoxia-inducible factor 1αand core binding factorα1, which play key roles in regulating bone healing. However, whether the accelerated bone healing is correlated with the expression of hypoxia-inducible factor 1αand core binding factorα1 is stil unknown. <br> OBJECTIVE:To construct rat models of brain injury, to compare the expression level of hypoxia-inducible factor 1αand core binding factorα1 in femoral fracture combined with cerebral trauma rats and simple femoral fracture rats, and to assess the influence of cerebral trauma on bone healing. <br> METHODS:Rats were randomly divided into blank group, simple femoral fracture group and femoral fracture combined with cerebral trauma group. At 1, 2, 3 and 5 weeks after modeling, rats were executed. Bone healing was evaluated using femoral fracture end X-ray score and hematoxylin and eosin staining at cal us tissues. Besides, the expression levels of hypoxia-inducible factor 1αand core binding factorα1 of three groups were determined with immunohistochemistry. <br> RESULTS AND CONCLUSION:Bone healing in the femoral fracture combined with cerebral trauma group was better than that of simple femoral fracture group. There was significant difference in the expression level of hypoxia-inducible factor 1αand core binding factorα1 between the simple femoral fracture group and femoral fracture combined with cerebral trauma group (P<0.05). At the same time, the level of simple femoral fracture group and femoral fracture combined with cerebral trauma group was significantly higher than that of blank group, and that in femoral fracture combined with cerebral trauma group was significantly higher than that of simple femoral fracture group (P<0.05). Results verified that the expression levels of hypoxia-inducible factor 1αand core binding factorα1 of rats with femoral fracture combined with cerebral trauma were significantly high, which may be the major reason why the bone healing was accelerated after fracture combined with brain injury.关键词
实验动物/骨及关节损伤模型/低氧诱导因子1α/核心结合因子α1/股骨/骨折/脑损伤/X射线/骨折愈合/大鼠/加速Key words
Tissue Engineering/Femur/Fractures, Bone/Fracture Healing分类
医药卫生引用本文复制引用
柏小金,徐林,骆旭东,刘福英,黄文良,郭元,马立坤,程小菊,柏猛..构建股骨骨折合并脑损伤模型大鼠低氧诱导因子1α和核心结合因子α1的表达[J].中国组织工程研究,2015,(18):2800-2806,7.基金项目
遵义市科技局资助项目[遵市科合社字(2011)19号]@@@@Funding:the Zunyi Municipal Science and Technology Bureau Project, No.(2011)19 （2011）
