中国组织工程研究Issue(18)：2838-2842,5.DOI:10.3969/j.issn.2095-4344.2015.18.009
构建不同周龄载脂蛋白E基因敲除动脉粥样硬化模型小鼠血脂和病理学观察
Serum lipid levels and pathological observation of apolipoprotein E knockout mice with atherosclerosis at different weeks of age
摘要
Abstract
BACKGROUND:The formation of atherosclerotic lesions in apolipoprotein E knockout mice is similar to that of human systemic atherosclerosis, and apolipoprotein E knockout mice are ideal animals for current establishment of atherosclerosis models. <br> OBJECTIVE:To research the pathological process of atherosclerosis in apolipoprotein E knockout mice aged different weeks, and to explore the effect of different diets on the occurrence and development of atherosclerosis in apolipoprotein E knockout mice. <br> METHODS:Male apolipoprotein E knockout mice aged 8 weeks old were randomly divided into two groups, and fed with high fat diet and normal diet, respectively, for 8, 12, 16, 20, and 24 weeks. <br> RESULTS AND CONCLUSION:Serological detection revealed that serum total cholesterol, triglycerides and low density lipoprotein levels were significantly higher in different weeks of mice of high fat diet group than in the normal diet group (P<0.05), in a time-dependent manner. Gross and frozen oil red O staining showed that atherosclerotic plaque area of lumen was significantly larger in the high fat diet group than in the normal diet group (P<0.05), in a time-dependent manner. At this time, significant differences in plaque area of lumen at each week were detected between both groups (P<0.05). Apparent lipid plaque was visible in aorta at 16 weeks of high fat diet in mice. Results demonstrated that apolipoprotein E knockout mice of atherosclerosis were successful y established. The formation of lipid streaks and fiber hyperplasia was faster in high fat diet group than in the normal diet group.关键词
实验动物/心脏及血管损伤模型/载脂蛋白E基因敲除小鼠/动脉粥样硬化/高脂饮食/主动脉/油红O染色/国家自然科学基金Key words
Models/Animals/Atherosclerosis/Serum Lipid/Pathology分类
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谢佳,陈清杰,杨毅宁,马依彤,李晓梅,刘芬,陈邦党,翟慧,周韵..构建不同周龄载脂蛋白E基因敲除动脉粥样硬化模型小鼠血脂和病理学观察[J].中国组织工程研究,2015,(18):2838-2842,5.基金项目
国家自然科学基金项目(81160042，81460069)@@@@Funding:the National Natural Science Foundation of China, No.81160042,81460069 （81160042，81460069）
