中国组织工程研究Issue(23)：3686-3691,6.DOI:10.3969/j.issn.2095-4344.2015.23.014
人端粒酶反转录酶基因修饰脐带间充质干细胞移植治疗急性肾损伤
Human telomerase reverse transcriptase gene-modified umbilical cord mesenchymal stem cell transplantation for acute kidney injury
王天生 1张建军2
作者信息
- 1. 天津市第五中心医院肾内科，天津市 300451
- 2. 天津市第四中心医院神经外科，天津市 300451
- 折叠
摘要
Abstract
BACKGROUND:The human telomerase reverse transcriptase (hTERT) is one of preferred growth factors for regulating proliferation and directional differentiation, has multiple biological effects, and laids the foundation for geneticaly engineered immortalized stem cel lines. OBJECTIVE: To investigate the effect ofhTERT gene-modified umbilical cord mesenchymal stem cel transplantation on acute kidney injury induced by ischemia and reperfusion in rats. METHODS:The human umbilical cord mesenchymal stem cels were cultured in vitro. Rat models of acute kidney injury induced by ischemia and reperfusion were established. Rat models were randomly divided into three groups. Rats in the control group were injected with 1 mL L-DMEM medium through caudal vein. Rats in the negative transfection group were injected with 1 mL umbilical cord mesenchymal stem cel suspension after empty virus transfection through caudal vein. Rats in the hTERT transfection group were injected with 1 mL umbilical cord mesenchymal stem cel suspension after PLXSN-hTERT transfection through caudal vein. RESULTS AND CONCLUSION:At 3 and 28 days after transplantation, hematoxylin-eosin staining showed renal tubular damage score in the hTERT transfection group < negative transfection group < control group (P < 0.05). At 28 days after transplantation, the number of CM-Dil-positive cels in the hTERT transfection group > negative transfection group > control group (P < 0.05). At 1, 3, 14, and 28 days, serum creatinine and urea nitrogen levels in the hTERT transfection group < negative transfection group < control group (P < 0.05). The results confirm that hTERT gene-modified umbilical cord mesenchymal stem cel transplantation has a significant repair effect on acute kidney injury in rats.关键词
干细胞/移植/人端粒酶反转录酶/脐带脐血干细胞/基因修饰/脐带间充质干细胞/大鼠/急性肾损伤/修复/端粒酶反转录酶/干细胞移植/肾损伤Key words
Tissue Engineering/Stem Cells/Umbilical Cord Mesenchymal Stem Cells/Rats/Renal Injury分类
医药卫生引用本文复制引用
王天生,张建军..人端粒酶反转录酶基因修饰脐带间充质干细胞移植治疗急性肾损伤[J].中国组织工程研究,2015,(23):3686-3691,6.
