中国组织工程研究2015，Vol.19Issue(16)：2523-2528,6.DOI:10.3969/j.issn.2095-4344.2015.16.012
碳纤维增强纳米羟基磷灰石/聚酰胺66复合材料与动物体内骨组织的相容性
In vivo animal study on osteal histocompatibility of carbon fiber-reinforced nano-hydroxyapatite/polyamide 66 composites
鹿鸣 1张雪松 2徐辉 1胡文浩 3杨晓青1
作者信息
- 1. 解放军总医院骨科,北京市 100853
- 2. 总参管理保障部北极寺老干部服务管理局门诊部,北京市 100191
- 3. 山东聊城市人民医院骨科,山东省聊城市 252004
- 折叠
摘要
Abstract
BACKGROUND:Compared with hydroxyapatite materials and other nano-hydroxyapatite composites, carbon fiber-reinforced nano-hydroxyapatite/polyamide 66 composites have been significantly improved in the mechanical strength, toughness, elastic modulus and other aspects. It can be used for repairing bone defects of loading parts. OBJECTIVE:To investigate the biocompatibility of carbon fiber-reinforced nano-hydroxyapatite/polyamide 66 composites in bone tissues. METHODS:Eight Bama mini pigs were taken to establish models of thoracic vertebral defects and implanted with carbon fiber-reinforced nano-hydroxyapatite/polyamide 66 composites. At 8, 16 and 24 weeks after implantation,the animals were sacrificed, respectively, for bone mineral density detection and hematoxylin-eosin staining. Blood samples for kidney and liver function tests were taken before and 1 and 8 weeks after implantation. RESULTS AND CONCLUSION: Hematoxylin-eosin staining of bone samples showed that the materials could bond with the bone defect interface without rejection, and could induce osteogenesis of chondrocytes. At 8 weeks after surgery, the broken ends of cancelous bone closed and the composite material was wrapped by granulation tissues. At 16 weeks after surgery, granulation tissues were organized and new bone developed directly from fibroblast cels. The new bone tissues were nearly fused with the end of cancelous bone. At 24 weeks after surgery, new bone tissue became mature lamelar bone, and the end of cancelous bone was connected tightly with the composite material. Bone mineral density of the implanted vertebra showed an increase trend at 8, 16 and 24 weeks after implantation. Over time, the bone mass was increased. The liver and kidney function tests showed that there was no significant difference before and after implantation. It is preliminarily believed that the carbon fiber-reinforced nano-hydroxyapatite/polyamide 66 composite has excelent histocompatibility and bioactivity without hepatic toxicity and nephritic toxicity.关键词
生物材料/材料相容性/纳米羟基磷灰石/聚酰胺66/碳纤维/组织相容性/骨缺损/修复分类
医药卫生引用本文复制引用
鹿鸣,张雪松,徐辉,胡文浩,杨晓青..碳纤维增强纳米羟基磷灰石/聚酰胺66复合材料与动物体内骨组织的相容性[J].中国组织工程研究,2015,19(16):2523-2528,6.
