中国组织工程研究2015，Vol.19Issue(16)：2529-2533,5.DOI:10.3969/j.issn.2095-4344.2015.16.013
灌注法制备全肾脏脱细胞基质支架的体内生物相容性
In vivo biocompatibility of whole-kidney acellular matrix scaffolds prepared by perfusion method
摘要
Abstract
BACKGROUND:The whole-kidney acelular matrix scaffold in rats prepared by perfusion method is proved to have goodin vitro biocompatibility, butin vivo biocompatibility is stil unclear. OBJECTIVE:To produce a whole-kidney acelular matrix scaffold in rats by perfusion method and to evaluate the in vivo biocompatibility of the scaffold. METHODS:The whole-kidney acelular matrix scaffold in Wistar rats was prepared by perfusion method and evaluated with the folowing tests. (1) Acute toxicity test: mice were subject to intraperitoneal injection of whole-kidney acelular matrix scaffold extract liquid, normal saline and phenol. (2) Hemolytic test: Anticoagulant blood samples from New Zealand rabbits were mixed with whole-kidney acelular matrix scaffold extract liquid, normal saline and distiled water, respectively. (3) Pyrogen test: Whole-kidney acelular matrix scaffold extract liquid was injected into the ear vein of New Zealand rabbits. (4) Intracutaneous stimulation test: Whole-kidney acelular matrix scaffold extract liquid was injected subcutaneously into New Zealand rabbits for observing whether there was a skin stimulus response. (5) Subcutaneous implantation test: The whole-kidney acelular matrix scaffold was implanted subcutaneously into the back of New Zealand rabbits. RESULTS AND CONCLUSION:There was no cel residual in the whole-kidney acelular matrix scaffold preparedby perfusion method, and no acute systemic toxicity, hemolytic reaction, pyrogen response, and skin stimulus respons, indicating the scaffold has a good histocompatibility in the rabbits. These findings suggest that the whole-kidney acelular matrix scaffolds in Wistar rats prepared by perfusion method have goodin vivo biocompatibility.关键词
生物材料/材料相容性/脱细胞基质/生物相容性/组织工程/灌注法/肾脏分类
医药卫生引用本文复制引用
陈捷,杨镜秋,刘春晓..灌注法制备全肾脏脱细胞基质支架的体内生物相容性[J].中国组织工程研究,2015,19(16):2529-2533,5.基金项目
广东省医学科学技术研究基金(A2012394) （A2012394）
