中国组织工程研究2015，Vol.19Issue(16)：2598-2602,5.DOI:10.3969/j.issn.2095-4344.2015.16.026
囊胚培养液对小鼠胚胎的毒性:体外生殖辅助医疗器械安全性评价
Toxicity of blastula culture medium to mouse embryos: a safety evaluation ofinvitro assisted reproduction devices
韩倩倩 1尹艳云 2王涵 1冯晓明 1王春仁 1杨昭鹏1
作者信息
- 1. 中国食品药品检定研究院,医疗器械检定所,生物材料和组织工程室,北京市 100050
- 2. 中国科学院遗传与发育生物学研究所,北京市 100190
- 折叠
摘要
Abstract
BACKGROUND:The blastula culture medium can assist the development of zygote from the fertilized egg to the blast blastula. The safety and quality of blastula culture medium directly influences the quality of blastula. OBJECTIVE:To evaluate the effect of blastula culture medium on the development of mouse embryos. METHODS: In this study, B6D2F1 mice were used. The female mice were superovulated and mated with male B6D2F1 mice. One day later, the zygotes were colected and cultured in the M16 medium to 4-cel stage. Then, 4-cel stage embryos were transferred into the tested blastula culture medium (experimental group). After 5 days of culture, the forming rate of blastula was examined. Meanwhile, the M16 medium containing endotoxin was used to culture 1-cel mouse zygote (positive control group). The M16 medium with no embryo toxicity was used to culture 1-cel zygote (negative control group). RESULTS AND CONCLUSION:The formation rate of blastula was 0 in the positive group, 87.1% in the negative control group, and 87.3% in the experimental group. From the results, the tested blastula culture medium could assist the 1-cel zygote growing to the stage of blastula, and the formation rate of blastula was above 80%. The tested blastula culture medium had no toxicity to the mouse embryo.关键词
生物材料/材料相容性/囊胚培养液/体外生殖/医疗器械/胚胎毒性分类
医药卫生引用本文复制引用
韩倩倩,尹艳云,王涵,冯晓明,王春仁,杨昭鹏..囊胚培养液对小鼠胚胎的毒性:体外生殖辅助医疗器械安全性评价[J].中国组织工程研究,2015,19(16):2598-2602,5.
