中国组织工程研究2015，Vol.19Issue(19)：3017-3021,5.DOI:10.3969/j.issn.2095-4344.2015.19.011
丁香酚胚胎毒性评价:应用胚胎干细胞的实验模型
Embryotoxicity of eugenol based on a model of embryonic stem cell test
摘要
Abstract
BACKGROUND:As the pharmacological effect of eugenol constantly being discovered, its application in medical and food industry becomes wider. However, its toxicity studies have not established a complete database, especialy in the improvement of safety assessment of developmental toxicity and teratogenicity. OBJECTIVE:To establish a model of embryonic stem cel test to evaluate the embryotoxicity of eugenol. METHODS:Mouse fibroblasts (3T3) and mouse embryonic stem cels (E14TG2a) were culturedin vitro, and MTT test was performed to detect the cytotoxicity of 3T3 cels and E14TG2a cels with positive control 5-fluorouracil, negative control penicilin G and tested compound eugenol. The concentration of the tested compounds that inhibiting 50% viability of embryonic stem cels (IC50 E14TG2a) and 3T3 fibroblasts (IC50 3T3) was calculated. The hanging-suspension-adherent culture systems were used to induce embryonic stem cels into cardiomyocytes, and the concentration of tested compounds that caused 50% inhibition of differentiation of E14TG2a cels into cardiomyocytes (ID50 E14TG2a) was calculated. The embryotoxic potential of eugenol was classified by prediction model of the embryonic stem cel test. RESULTS AND CONCLUSION:The proliferations of E14TG2a and 3T3 cels were inhibited by eugenol, of which the IC50 3T3 and IC50 E14TG2a values were (3.613±0.192) and (1.799±0.131) mg/L. The differentiation of E14TG2a was also inhibited by eugenol, of which the ID50 E14TG2a was (3.501±0.158) mg/L. Eugenol was evaluated as a chemical compound with strong embryotoxicity by the model of embryonic stem cel test.关键词
干细胞/胚胎干细胞/丁香酚/胚胎发育/胚胎干细胞实验模型/胚胎毒性/毒理学/心肌细胞/国家自然科学基金分类
医药卫生引用本文复制引用
李付贵,陈京,程伟民,季明芳..丁香酚胚胎毒性评价:应用胚胎干细胞的实验模型[J].中国组织工程研究,2015,19(19):3017-3021,5.基金项目
国家自然科学基金青年基金项目(81202604) （81202604）
中国博士后科学基金(2014M562244) （2014M562244）
广东省自然科学基金(2014A030310013) the National Natural Science Foundation of China, No. 81202604 （2014A030310013）
the China Postdoctoral Foundation, No. 2014M562244 （）
the Natural Science Foundation of Guangdong Province, No. 2014A030310013 （）
