中国组织工程研究2015，Vol.19Issue(20)：3200-3204,5.DOI:10.3969/j.issn.2095-4344.2015.20.016
深低温保存兔肢体不同方法复温后许旺细胞的活性
Different thawing methods for cryopreserved rabbit limbs: activity of Schwann cells after thawing
朱泽兴 1王丹 2张树明 1赵艳东 3樊凯彬 4赵永军 5李鸿江 6李波 7乔林1
作者信息
- 1. 解放军第二炮兵总医院,骨科,北京市 100088
- 2. 解放军第二炮兵总医院,药学部,北京市 100088
- 3. 山西省临汾市第四人民医院,山西省临汾市 041000
- 4. 邯郸市中心医院,河北省邯郸市 056001
- 5. 解放军武警部队后勤学院附属医院骨科,天津市 300162
- 6. 北京丰台铁营医院骨科,北京市 100078
- 7. 天津泰达医院骨科,天津市 300457
- 折叠
摘要
Abstract
BACKGROUND:According to the characteristics of nerve regeneration, the integrity of Schwann cels as regenerative scaffold is very important. Therefore, to save the Schwann cel structure and activity is most important for nerve cryopreservation. OBJECTIVE: To investigate the optimal thawing procedures for the survival of peripheral nerves in cryopreserved rabbit limbs. METHODS:Forty hindlimbs from New Zealand White rabbits were randomized into four groups: blank, fast-thawing, slow-thawing and control groups. The control group was subjected to perfusion with protective agent. The severed limbs in fast-thawing and slow-thawing groups were perfused with protective agent folowed by gradient cryopreservation and storage in liquid nitrogen for 72 hours. Then, the slow-thawing group was given gradient thawing procedures, and the fast-thawing group was subjected to fast thawing in 37℃ water bath. After thawing, the tibial nerves were harvested for light microscope, electron microscope, immunofluorescence and confocal microscope observation. SPSS17.0 was used for statistical analysis. RESULTS AND CONCLUSION:No significant difference was found between the blank and control groups. Nerve fiber breakage, axonal damage and mitochondrial sweling were severer in the fast-thawing and slow-thawing groups than the control and blank groups. In addition, the fast-thawing group was superior to the slow-thawing group. These findings indicate that the fast-thawing method has the minimal effects on the survival of nerve fibers in cryopreserved rabbit limbs.关键词
组织构建/组织工程/深低温保存/断肢再植/神经/复温分类
医药卫生引用本文复制引用
朱泽兴,王丹,张树明,赵艳东,樊凯彬,赵永军,李鸿江,李波,乔林..深低温保存兔肢体不同方法复温后许旺细胞的活性[J].中国组织工程研究,2015,19(20):3200-3204,5.
