中国组织工程研究2015，Vol.19Issue(21)：3309-3316,8.DOI:10.3969/j.issn.2095-4344.2015.21.006
两种人工合成骨形态发生蛋白活性多肽的骨诱导能力
Two kinds of synthesized bone morphogenetic protein active polypeptides: evaluation of osteoinductive activity
王硕 1甘少磊 2谢惠敏 3任卫卫 1李南 4宋光泽 2韦兴2
作者信息
- 1. 解放军医学院,北京市 100853
- 2. 解放军总医院第一附属医院骨科,北京市 100048
- 3. 博恩康生物科技有限公司,北京市 100085
- 4. 中国地质大学材料科学与工程学院,北京市 100083
- 折叠
摘要
Abstract
BACKGROUND:According to the core functional zone of amino acid sequence of the osteoinduction in bone morphogenetic proteins, our research group synthesized bone morphogenetic protein (BMP) active polypeptides Ⅰ and Ⅱ by artificial solid-state synthesis method. OBJECTIVE: To evaluate the osteoinductive ability of BMP active polypeptides Ⅰ and Ⅱ in animals. METHODS:Forty-two Sprague-Dawley rats were randomly divided into seven groups, and respectively implanted with hydroxyapatite/polylactic acid carrying 0.2, 0.4, 0.8 g/L BMP active polypeptides I, hydroxyapatite/polylactic acid carrying 0.2, 0.4, 0.8 g/L BMP active polypeptides Ⅱ, and hydroxyapatite/polylactic acid alone. At 3 and 5 weeks postoperatively, X-ray, CT and histological detection were conducted to evaluate osteoinductive conditions in the seven groups. RESULTS AND CONCLUSION:At 3 and 5 weeks postoperatively, there were better local osteoinductive effects in the groups hydroxyapatite/polylactic acid carrying BMP active polypeptides Ⅰ and Ⅱ than the group of hydroxyapatite/polylactic acid, indicating both two kinds of BMP active polypeptides possessed a certain osteoinductive ability. Moreover, this osteoinductive ability became stronger with time. At 5 weeks postoperatively, the osteoinductive effect in the 0.4 and 0.8 g/L BMP active polypeptides I groups was better than that in the 0.2 g/L BMP active polypeptides I group and the 0.2, 0.4 and 0.8 BMP active polypeptides Ⅱ groups (P < 0.05). In addition, there was no difference in the osteoinductive effect of 0.4 and 0.8 g/L BMP active polypeptides I groups. These results indicate that BMP active polypeptides I has a stronger osteoinductive ability than BMP active polypeptides Ⅱ.关键词
生物材料/材料相容性/骨形态发生蛋白/活性多肽/骨诱导/骨修复材料/羟基磷灰石/聚乳酸分类
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王硕,甘少磊,谢惠敏,任卫卫,李南,宋光泽,韦兴..两种人工合成骨形态发生蛋白活性多肽的骨诱导能力[J].中国组织工程研究,2015,19(21):3309-3316,8.
