中国实用神经疾病杂志Issue(2)：41-43,3.
姜黄素对阿尔茨海默病细胞模型miRNA106a 的影响
Effects of curcumin on miRNA106a in the cell model of Alzheimer's disease
乔娜娜 1王运良2
作者信息
- 1. 潍坊医学院 潍坊 261053
- 2. 解放军第148中心医院神经内科 淄博 255300
- 折叠
摘要
Abstract
Objective To investigate the relationship between curcumin and miR‐106a in Alzheimer’s disease.Methods PC12 cells were randomly divided into 5 groups, namely, normal control group, model group (cultured with 4 μg/mL Abeta1‐42 for 24 hours), anti‐miR‐106a group ( transfected with anti‐miR‐106a), curcumin group (adding 20 μmol/L curcumin) and coaction group (adding curcumin and anti‐miR‐106a).The miR‐106a content of each group was detected by RT‐PCR and APP content was detected by Western blotting.Results RT‐PCR showed that the concentration of miR‐106a decreased significantly in model group and anti‐miR‐106a group but increased markedly in curcumin group compared with normal control group.Western‐blotting showed that the APP content increased significantly in APP model group and anti‐miR‐106a group but decreased markedly in curcumin group compared with normal control group.Conclusion Curcumin maybe positively regulate miR‐106a in alzheimer’s disease.关键词
姜黄素/miR-106a/anti-miR-106a/阿尔茨海默病Key words
Curcumin/miR-106a/Anti-miR-106a/Alzheimer’s disease分类
医药卫生引用本文复制引用
乔娜娜,王运良..姜黄素对阿尔茨海默病细胞模型miRNA106a 的影响[J].中国实用神经疾病杂志,2015,(2):41-43,3.
