癌变·畸变·突变Issue(3)：171-174,179,5.DOI:10.3969/j.issn.1004-616x.2014.03.003
Toll样受体基因多态性与EBV感染相关胃癌的易感性
Toll-like receptors gene polymorphism and susceptibility to EBV-associated gastric carcinoma
摘要
Abstract
OBJECTIVE: To explore the relationship of Toll-like receptors 2 (TLR2) and TLR4 Thr399Ile gene polymorphism and the susceptibility to EBV-associated gastric carcinoma (EBVaGC) and EBV-negative gastric carcinoma(EBVnGC). METHODS:TLR2 gene promoter region-196 to-174 del polymorphism was detected by using the allele-specific polymerase chain reaction in 52 EBVaGCs,157 EBVnGCs and 94 normal controls. TLR4 gene Thr399Ile genotype and allele distribution were evaluated by PCR and restriction fragment length polymorphism (PCR-RFLP) in 50 EBVaGCs,67 EBVnGCs and 71 normal controls. The relationship between gene polymorphism in TLR2 and TLR4 coding genes and the susceptibility to EBVaGC and EBVnGC was analysed. RESULTS:There was no significant 2difference ( =3.180,P=0.075) between gastric cancer group and control group in genotype distribution of TLR2(-196 to-174 del),but the del allele frequency of gastric cancer group was obviously higher than that of control group 2( =4.875,P=0.027),The risk of developing gastric carcinoma in del allele carriers was significantly higher than that in controls(OR=1.491,95%CI=1.045-2.126). TLR2 (-196 to-174 del) genotype and the frequency of del allele showed no 2significant difference in EBVaGC and EBVnGC group ( =0.05,P=0.867). TLR4 gene Thr399Ile locus mutation was not found in EBVaGC,EBVnGC or normal control groups. The genotype distribution and allele frequency also revealed no statistical differences . CONCLUSION:TLR2-196 to-174 del alleles could be risk factors for gastric carcinoma,butχχχits effects were not different in EBVaGC and EBVnGC. There was no obvious correlation in the genotype distribution of TLR2(-196 to-174 del) and TLR4 gene Thr399Ile and EBVaGC genetic susceptibility.关键词
Tol样受体/胃癌/EB病毒/基因多态性Key words
Toll-like receptor/gastric carcinoma/EBV/gene polymorphism分类
医药卫生引用本文复制引用
赵真真,石源源,刘颂,王笑峰,罗兵..Toll样受体基因多态性与EBV感染相关胃癌的易感性[J].癌变·畸变·突变,2014,(3):171-174,179,5.基金项目
国家自然科学基金(30970157)，山东省自然科学基金(ZR2011CM016) （30970157）
