癌变·畸变·突变Issue(3)：189-192,4.DOI:10.3969/j.issn.1004-616x.2014.03.007
Survivin、NF-кB和STAT3 mRNA在宫颈癌中的表达及其临床意义
The expression and significance of Sur vivin, NF-κB and STAT3 mRNA in cer vical cancer
常玉珍 1李秀梅 2封敏 3高崎1
作者信息
- 1. 新疆维吾尔自治区中医医院妇科，新疆乌鲁木齐 830099
- 2. 新疆医科大学形态中心，新疆 乌鲁木齐 830011
- 3. 新疆医科大学附属肿瘤医院检验科，新疆 乌鲁木齐 830000
- 折叠
摘要
Abstract
OBJECTIVE:To test expressions of Survivin,NF-κB and STAT3 in 40 cervical carcinoma tissue and 30 normal cervical tissue samples. The correlation between Survivin, NF-κB and STAT3 expression level and the clinical factors and pathology classification were analyzed,and the relationship and mechanism of Survivin,NF-κB and STAT3 in cervical carcinoma development were studied. METHODS:Trizol one step method was used to extract total RNA from both cervical carcinoma and normal tissues,mRNA reverse transcriptase cDNA,the polymerase chain reaction (RT-PCR half quantitative) method was used to measure Survivin, NF-κB and STAT3 mRNA expressions in cervical carcinoma and normal tissues. Further analysis of correlation between Survivin, NF-κB and STAT3 mRNA expression and the clinical pathological factors were performed. RESULTS:Survivin, NF-κB and STAT3 mRNA expressions in carcinoma tissue were significantly higher than the normal tissue (P<0.05). Survivin,NF-κB and STAT3 mRNA expression inⅡb-Ⅲa stage was significantly higher thanⅠ-Ⅱa stage (P<0.01). The expression of Survivin mRNA in patients with lymph node metastasis was higher than those with no lymph node metastasis (P<0.01). Survivin expression was related to STAT3 expression (r=0.483,P<0.05) and to NF-κB expression (r=0.377,P<0.05). CONCLUSION:Survivin,NF-κB and STAT3 expressions were upregulated in cervical carcinoma. All of these were closely related to the development and progression of cervical carcinoma.关键词
宫颈癌/Survivin/NF-κB/STAT3Key words
cervical carcinoma/Survivin/NF-κB/STAT3分类
医药卫生引用本文复制引用
常玉珍,李秀梅,封敏,高崎..Survivin、NF-кB和STAT3 mRNA在宫颈癌中的表达及其临床意义[J].癌变·畸变·突变,2014,(3):189-192,4.
