癌变·畸变·突变Issue(4)：298-300,304,4.DOI:10.3969/j.issn.1004-616x.2014.04.013
Survivin shRNA干扰对食管癌细胞CDK4 mRNA表达的影响
Expression of Sur vivin shRNA interference on CDK4 in esophageal ECA109 cells
摘要
Abstract
To study Survivin shRNA interference on CDK4 mRNA and protein levels of esophageal cells ECA109. METHODS:Survivin shRNA interference technology was applied to liposomes transfection esophageal ECA109 cells,RT-PCR and Western blot methods was applied to evaluate genes expression of Survivin and CDK4. Flow cytometry was used to detect the proliferation of cell lines index changes after ECA109 esophageal cell transfection. RESULTS:After Survivin shRNA transfection of esophageal ECA109 cell,Survivin mRNA and protein expressions were obviously down-regulated. CDK4 mRNA expression was also down-regulated. Cell ratio was increased in G2 phase but decrease in S phase,with the cell cycle blocked. CONCLUSION:Survivin was effective on adjusting CDK4 gene expression at mRNA level. The role of silencing Survivin down-regulated expression of CDK4 mRNA,suggesting that Survivin could be a new gene regulatory factor in the tumor signal control network.关键词
Survivin/食管癌/短发夹RNA/RNA干扰/CDK4Key words
Survivin/esophageal cancer/short hairpin RNA/RNA interference/CDK4分类
医药卫生引用本文复制引用
李秀梅,李卉,姜孝芳,陈艳,湛宏鸣,李惠武..Survivin shRNA干扰对食管癌细胞CDK4 mRNA表达的影响[J].癌变·畸变·突变,2014,(4):298-300,304,4.基金项目
国家自然科学基金(81360304) （81360304）
