癌变·畸变·突变Issue(5)：374-377,381,5.DOI:10.3969/j.issn.1004-616x.2014.05.012
邻苯二甲酸二（2-乙基己）酯对大鼠卵巢组织凋亡基因和癌基因表达水平的影响
Effects of di(2-ethylhexyl) phthalate on expression of apoptosis genes and oncogenes in rat ovary
摘要
Abstract
OBJECTIVE: To investigate the reproductive toxicity of di(2-ethylhexyl) phthalate (DEHP), and to assess the expressions of apoptosis genes and oncogenes after DEHP treatment in female SD rats. METHODS:Female SD rats were treated with different doses of DEHP (corn oil group used as control,DEHP treatment including 100 mg/kg,500 mg/kg,1 500 mg/kg) for 6 weeks. The expression levels of apoptosis genes(Bcl-2,Caspase-3,Caspase-8,Caspase-9) and oncogenes (c-fosand k-ras) mRNA were determined by real-time quantitative PCR.RESULTS:Bcl-2 expression level at DEHP 1 500 mg/kg group was significantly decreased in comparison with control group(P<0.01). The expression levels ofCaspase-3,Caspase-8 andCaspase-9 mRNA were significantly increased in rat ovarian tissue after DEHP treatment (P<0.05 orP<0.01). c-fos mRNA expression levels showed no significant difference compared with control. k-ras mRNA expression levels increased significantly in DEHP 500 mg/kg and 1 500 mg/kg treatment groups(P<0.01).CONCLUSION:DEHP could activate Caspase apoptosis pathway through the mitochondrial pathway,resulting in apoptosis of ovarian cells,leading to further damage of ovarian function and reproductive endocrine function. DEHP could also activate oncogene expression,therefore DEHP could possess potential carcinogenesis risk.关键词
邻苯二甲酸二(2-乙基己)酯/生殖毒性/凋亡基因/癌基因Key words
di(2-ethylhexyl) phthalate/reproductive toxicity/apoptosis genes/oncogenes分类
医药卫生引用本文复制引用
谭琴,秦逍云,徐新云,吴德生,杨细飞,黄海燕,周丽,黄新凤..邻苯二甲酸二（2-乙基己）酯对大鼠卵巢组织凋亡基因和癌基因表达水平的影响[J].癌变·畸变·突变,2014,(5):374-377,381,5.基金项目
广东省科技计划项目(2013B021800032) （2013B021800032）
