癌变·畸变·突变Issue(5)：382-385,4.DOI:10.3969/j.issn.1004-616x.2014.05.014
溪黄草袋泡茶的急性毒性和遗传毒性研究
Study on the acute toxicity and genetic toxicity of teabags rabdosia serra rabdosia serra
陈秀娟 1黄俊明 1李欣 1陈美芬 1李庆 1梁杨盛 1易资梅2
作者信息
- 1. 广东省疾病预防控制中心，广东广州511430
- 2. 广东省生物制品与药物研究所，广东 广州 510440
- 折叠
摘要
Abstract
OBJECTIVE: To investigate the acute toxicity and genetic damage in experimental animals. METHODS:In the acute toxicity study,mice were intragastrically administered by 21.5,10.0,4.64,2.15 g/kg rabdosia serra teabags groups and calculated the median lethal dose (LD50) by Horn method.In Ames test,set 5 000, 1 000,200,40,8μg rabdosia serra teabags per plate.In mice bone marrow cell micronucleus and mice sperm abnormality test,2.50,5.00 and 10.0 g/kg rabdosia serra teabags groups.RESULTS:The acute oral toxicity in rabdosia serra teabags was more than 21.5 g/kg.In Ames test with and without S9 metabolic activation,the reverse mutations of typhimurium TA97, TA98,TA100,TA102 were less than double compared with natural mutations,and no dose-response relationship was found.The micronucleus rates and sperm abnormality rates in all doses showed no significant difference from the negative control (P>0.05).CONCLUSION:Under these experimental conditions, rabdosia serra teabags was shown to be a non-toxic substance.It did not cause genetic toxicity.关键词
溪黄草/急性毒性/Ames试验/微核试验/精子畸形试验/遗传毒性Key words
rabdosia serra/acute toxicity/ames test/micronucleus test/sperm abnormality test/genetic toxicity分类
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陈秀娟,黄俊明,李欣,陈美芬,李庆,梁杨盛,易资梅..溪黄草袋泡茶的急性毒性和遗传毒性研究[J].癌变·畸变·突变,2014,(5):382-385,4.
