癌变·畸变·突变Issue(6)：412-418,7.DOI:10.3969/j.issn.1004-616x.2014.06.003
毛蚶蛋白抗肿瘤活性研究
The antitumor activity of Arca subcrenata protein
摘要
Abstract
OBJECTIVE: To isolate the active component of Arca subcrenata protein,investigate the antitumor and immune activities in vitro. METHODS:The Arca subcrenata protein active component was extracted by ice water and ammonium sulfate precipitation. Cytotoxicity in human rectal concinoma HT-29 and other cell lines treated with Arca subcrenata protein active component of 50,100,200,400μg/mL for 24,48 and 72 h. Morphology changes in living cells were examined under the inverted phase contrast microscope. Cellular and nuclear morphological changes in human tumor cells treated with the Arca subcrenata protein active component was studied by Giemsa staining. Cell cycle and apoptosis in HT-29 cells were determined by flow cytometry. The growth of mice macrophage RAW264.7 treated the Arca subcrenata protein active component was detected by MTT assay. The phagocytosis of mice macrophage RAW264.7 treated with Arca subcrenata protein active component was determined by neutral red. The NO production by RAW264.7 was detected by Griess method. RESULTS:Arca subcrenata protein active component inhibited the survival of many tumor cell lines in concentration and time-dependent manners. Arca subcrenata protein active component induced cell were cycle arrest at G2-M cycle phases and apoptosis in HT-29 cells. It also showed that multinucleated cells were increased. Under the concentration of 50-100 μg/mL,Arca subcrenata protein active component could stimulate the growth of RAW264.7 cells,enhance phagocytosis and NO production activity. CONCLUSION:Arca subcrenata protein active component demonstrated anti-tumor activities due to its immune activation and the fact that it induced cell cycle arrest at G2-M cycle phases and apoptosis.关键词
毛蚶/蛋白质/抗癌/免疫激活/G2-M期阻滞/凋亡Key words
Arca subcrenata/protein/antitumor/immune activation/G2-M cell cycle arrest/apoptosis分类
医药卫生引用本文复制引用
付莹,冯婷,赵晨,田佳鑫,林华英,孙震晓..毛蚶蛋白抗肿瘤活性研究[J].癌变·畸变·突变,2014,(6):412-418,7.基金项目
山东省科技发展计划项目(2011YD19007)，北京中医药大学自主选题项目(2014JYBZZ-XS097) （2011YD19007）
