癌变·畸变·突变Issue(6)：428-433,6.DOI:10.3969/j.issn.1004-616x.2014.06.006
纳米银对中国仓鼠肺成纤维细胞的细胞毒性和遗传毒性
Cytotoxicity and genotoxicity of silver nanopar ticles on the Chinese hamster lung fibroblast cell line
摘要
Abstract
OBJECTIVE: To study the cyto-and genotoxicities of silver nanoparticles (silver-NPs) on Chinese hamster lung fibroblast cell line (CHL) and explore their possible mechanisms. METHODS:CHLs were exposed to gradient concentrations of silver-NPs 2.5,5.0,10.0,20.0,40.0 and 80.0μg/mL,then MTT assay was employed to reveal the IC50 which was used to evaluate the cytotoxicity potential of silver-NPs. Cell cycle analysis was performed with PI dye,and proliferation indexes were calculated as well. Apoptosis was detected with Annexin V FITC-PI dye,morphology changes after exposure and forward scatter channel (FSC) and side scatter channel(SSC) were recorded simultaneously. Chromosomal aberrations were analyzed by reading slides with metaphases stained by Giemsa. RESULTS:Cytotoxicity on CHL was positively associated with the concentration of silver-NPs (r=0.804,P<0.05), IC50 was 21.68 μg/mL. Morphology changes were clearly visible after CHLs were treated with 22.0 μg/mL silver-NPs for 24 h. Significantly enhanced SSC signal existed in CHL populations after periods of exposure to silver-NPs,which implied uptake of silver-NPs. CHL cycle was found to be arrested in G2/M phase,and cell proliferation index as well as G0/G1 phase,S phase were significantly changed compared with vehicle control (P<0.01). Furthermore,apoptosis was found to be significantly increased after 22.0 μg/mL silver-NPs exposure for 24 h (P<0.01). Silver-NPs exposure also induced CHL chromosome structure aberration with more than 5% incidence at all tested concentrations (P<0.01). Tetraploid in high and middle concentrations-treated groups were 10 times higher than the vehicle control(P<0.01). CONCLUSION:Silver-NPs showed significant cytotoxicity and genotoxicity in CHL,where mitochondrial dysfunction and apoptosis might play important roles.关键词
纳米银/中国仓鼠肺成纤维细胞/细胞毒性/遗传毒性/细胞周期/凋亡Key words
silver nanoparticle/Chinese hamster lung fibroblast cell/cytotoxicity/genotoxicity/cell cycle/apoptosis分类
医药卫生引用本文复制引用
于永生,施畅,徐丽明,韩刚,尹纪业,马华智,丁日高..纳米银对中国仓鼠肺成纤维细胞的细胞毒性和遗传毒性[J].癌变·畸变·突变,2014,(6):428-433,6.基金项目
重大新药创制科技重大专项基金(2013ZX09302303，2012ZX09301-001-008)。 （2013ZX09302303，2012ZX09301-001-008）
