癌变·畸变·突变Issue(1)：1-5,5.DOI:10.3969/j.issn.1004-616x.2015.01.001
脱氧雪腐镰刀菌烯醇致小鼠畸形骨骼组织差异表达基因及相关通路分析
Differential gene expression and related pathways of skeletal malformations induced by deoxynivalenol in mice
摘要
Abstract
OBJECTIVE:To screen the differential gene expression and related pathways of fetal skeletal malformations induced by deoxynivalenol(DON) in mice,and to explore the mechanism of deformities induced by DON at the molecular level.METHODS:30 pregnant mice were randomly divided into 3 experimental groups and 2 control groups,6 in each group. DON injection was performed from days 7 to 10 of pregnancy by intraperitoneal injection into pregnant mice. At GD 18,all mice were killed under isoflurane anesthesia,and vertebral bone tissue of fetuses were collected. Total RNA of vertebral bone tissues was extracted and cDNA was obtained by RT-PCR. Using whole genome microarray technology,the gene expression profiles and the pathways of the rat vertebral bone tissue were studied. Analysis of the function of differentially expressed genes was performed by using software of gene ontology. Screening signaling pathways of differentially expressed genes was done by DAVID database. The results were verified by fluorescence quantitative PCR(qPCR) technology. RESULTS:Microarray analysis showed that 282 genes,including 148 down-regulated and 134 up-regulated genes,were abnormally expressed in fetal vertebral bones after maternal DON exposure. 153 pathways were related to the differentially expressed genes. The relative quantitative results ofqPCR were consistent w ith t he r esults o f g ene c hip t est i nFbn1,Co19a2,Papss2,Pax1,Runx2 a ndPthlh g enes.CONCLUSION: There were differential gene expressions in deformed fetal skeleton between deoxynivalenol-treated rats and normal rats, involving multiple signaling pathways. The differentially expressed genes and signaling pathways may be related to the molecular mechanisms of deformities induced by DON.关键词
脱氧雪腐镰刀菌烯醇/骨骼畸形/基因芯片/差异表达基因/传导通路Key words
deoxynivalenol/skeletal d eformities/gene c hip/differentially e xpressed g enes/signaling p athways分类
生物科学引用本文复制引用
李岩,赵英会,庄东明,李晓霞,于爱莲..脱氧雪腐镰刀菌烯醇致小鼠畸形骨骼组织差异表达基因及相关通路分析[J].癌变·畸变·突变,2015,(1):1-5,5.基金项目
山东省科技攻关项目(2007GG30002016)；国家传染病重大专项合作项目(2009ZX10004-216)；山东省自然科学基金(ZR2013HL060) （2007GG30002016）
