癌变·畸变·突变Issue(3)：168-171,176,5.DOI:10.3969/j.issn.1004-616x.2015.03.002
SOX30基因在结直肠癌中的表达与甲基化分析
The expression and methylation ofSOX30 gene in colorectal cancer
摘要
Abstract
OBJECTIVE:To analyze the expression and methylation of SOX30 gene in colorectal cancer cell lines and colorectal cancer tissues.METHODS:ThemRNA expression and methylation ofSOX30 were evaluated by reverse transcription-polymerase chain reaction (RT-PCR) andmethylation specific PCR (MSP). Demethylation experiment with 5-Aza-CdR was used to confirm the regulation ofSOX30 expression by DNA hypermethylation. The methylation ofSOX30 was further confirmed by sodium bisulfite DNA sequencing (BSP).RESULTS:Hypermethylation ofSOX30 gene was found in all the colorectal cancer cell lines,with reducedmRNAexpression. The mRNA expression ofSOX30 increased in colorectal cancer cell lines treated with 5-Aza-CdR,indicating thatSOX30 expression was regulated by DNA methylation.SOX30 hypermethylation was found in most of the colorectal cancer tissues (79.6%) ,but not in tumor adjacent tissues (20%). After investigating possible associations betweenSOX30 methylation and clinicopathologic features,we found thatSOX30 hypermethylation was associated with histological type,but not correlated to gender,age,smoking,clinical TNM stages and Ducks stages.CONCLUSION:Down-regulation ofSOX30 by hypermethylation was always found in colorectal cancer cell lines,and its hypermethylation was obviously associated with histological type,suggestingSOX30 playing an important role in the carcinogenesis of colorectal cancer.关键词
SRY盒包含基因30/DNA甲基化/结直肠癌/甲基化特异性PCR/硫化测序PCRKey words
SOX30/DNA methylation/colorectal cancer/methylation specific PCR/bisulfite squencing PCR分类
医药卫生引用本文复制引用
史荣辉,韩飞,董严,张明谦,姜晓,尹俐,刘文斌,曹佳,刘晋祎..SOX30基因在结直肠癌中的表达与甲基化分析[J].癌变·畸变·突变,2015,(3):168-171,176,5.基金项目
国家自然科学基金项目(81172714) （81172714）
