安徽大学学报（自然科学版）Issue(4)：94-101,8.DOI:10.3969/j.issn.1000-2162.2014.04.015
部分野生杜鹃杂交授粉后花粉管生长状况分析
Analysis of pollen tube growth after pollination in some wild Rhododendron
摘要
Abstract
Rhododendron simsii and Rhododendron pulchrum as maternal parent were crossed with 15 wild Rhododendron species. In order to make clear the interspecific cross compatibility and pre -fertilization barriers, the growth of pollen tube on the stigma and into the style was observed under the epifluorescence microscopy. The results showed that pollens of all cross combinations could be attached and germinated on stigmas of R. simsii and R. pulchrum, however pollen tube growth and the ratio of abnormal pollen tubes were different due to varied male parents. Different abnormalities of arrested pollen tube tips have been detected, including colied, spiralling, swollen, burst, heavy callose deposition on the wall and so on. The growth rate of pollen tube was more rapidly among cross combinations with better cross compatibility such as self - pollination and intraspecific hybridization of R. simsii, and their pollen tubes reached the bottom of style canal more than the cross combinations with worse cross compatibility. Different regions in the style canal and the ovary loculus for expression of pollen tube arrest have been found according to the different crosscombinations. R. simsii as maternal parent, pollen tube of all cross combinations reached the bottom of style canal, although pollen tube abnormality exited in all cross combinations. R. pulchrum as maternal parent, pollen tube growth of several cross combinations was inhibited in upper and lower style canal, and the ratio of pollen tube abnormality was higher. The results indicated R. simsii as maternal parent had better cross compatibility than R. pulchrum. In addition, abnormalities types of arrested pollen tube tips had no concern with cross compatibility, even if inbred combination had abnormal situation of pollen tubes, but emergence ratio of abnormal pollen tube was related to cross compatibility.关键词
杜鹃/花粉管/杂交亲和性Key words
Rhododendron/pollen tube/cross compatibility分类
农业科技引用本文复制引用
耿兴敏,张超仪,尹增芳..部分野生杜鹃杂交授粉后花粉管生长状况分析[J].安徽大学学报（自然科学版）,2014,(4):94-101,8.基金项目
江苏省自然科学基金资助项目(BK2011820) （BK2011820）
江苏省高校优势学科建设工程资助项目(PAPD) （PAPD）
