安徽大学学报（自然科学版）Issue(6)：89-95,7.DOI:10.3969/j.issn.1000-2162.2014.06.014
应用溴甲酚紫指示剂检测贵州省稻瘟病菌对稻瘟灵的敏感性
The sensitivity test of Magnapothe grisea to isoprothiolane by bromocresol purple approach in Guizhou Province
摘要
Abstract
The Magnapothe grisea’s sensitivity was tested on isoprothiolane by bromocresol purple approach from Guizhou.After a period of time,the researchers found that the Magnapothe grisea disk ’s color will change with the isoprothiolane ’s concentration in medium which contained indicator and isoprothiolane.When the concentration is lower than 4 mg · mL-1 ,it is purple and the color depth is decreased with the isoprothiolane concentration increased.The concentration of 4 mg · mL-1 presents orange red and the inhabitation ratio reached 80 percent.The results showed that the EC50 of the strains to isoprothiolane ranged from 1.41-6.26 mg·mL-1 ,the sensitive base-line of Magnapothe grisea’s isolates to isoprothiolane was 3.283 33 mg·mL-1 in Guizhou,most of isolates were low resistant to isoprothiolane.The sensitivity of strains belonging to different Magnapothe grisea’s to isoprothiolane had time and space differences.关键词
稻瘟病菌/稻瘟灵/敏感性测定/溴甲酚紫Key words
Magnapothe grisea/isoprothiolane/sensitivity test/bromocresol purple分类
农业科技引用本文复制引用
郭真香,李明..应用溴甲酚紫指示剂检测贵州省稻瘟病菌对稻瘟灵的敏感性[J].安徽大学学报（自然科学版）,2014,(6):89-95,7.基金项目
公益性行业(农业)科研专项(200903033-08) （农业）
