安徽工程大学学报Issue(2)：3-7,5.
重组毕赤酵母产匍枝根霉内切葡聚糖酶Ⅱ的分离纯化及其酶学性质研究
Separation purification and enzymatic properties of endoglucanase Ⅱ in pichia pastoris from rhizopus stolonifer
摘要
Abstract
The endoglucanase Ⅱ(egⅡ )of Rhizopus stolonifer has been expressed in Pichia pastoris.The high-level expression of recombinant endoglucanaseⅡ was achieved through high-density fermentation of recombinant Pichia pastoris.Ni column was used to purify the expressed product,the specific activitiy of purified egⅡ has been determined,and its molecular mass was 38 kDa.The results of enzyme character-ization showed that the purified egⅡ has a optimum catalytic activity at 55 ℃ and pH 5.0.The catalytic activity of egⅡ could be enhanced by metal ion Mn2+,Zn2+ and Fe2+,but strongly inhibited by Cu2+ at a high concentration (5 mM),while it has no effects by Mg2+,Co2+,Na+ and K+.The determined Km value of egⅡ was 2.04 mg/ml by using sodium carboxymethyl cellulose (CMC-Na)as the substrate.关键词
匍枝根霉/内切葡聚糖酶/分离纯化/酶学性质Key words
rhizopus stolonifer/endoglucanase/separation and purification/enzyme characterization分类
生物科学引用本文复制引用
程伟,汤斌,李松..重组毕赤酵母产匍枝根霉内切葡聚糖酶Ⅱ的分离纯化及其酶学性质研究[J].安徽工程大学学报,2015,(2):3-7,5.基金项目
国家自然科学基金资助项目(31270135) （31270135）
