安徽工程大学学报Issue(2)：29-33,5.
桑叶多糖的分离纯化及其抗凝血活性的研究
Isolation,purification and anticoagulant activity of the polysaccharide from the leaves of mulberry
摘要
Abstract
To study different components of mulberry polysaccharides and their anticoagulant activity,the crude mulberry polysaccharides were extracted by water and precipitated by alcohol.The crude polysac-charides were decolorized and deproteinized by polyamide.Five water-soluble polysaccharides (MPS-1 , MPS-2a,MPS-2b,MPS-2c and MPS-3)were purified by DEAE sepharose CL-6B chromatograph column and sepharose CL-6B chromatograph column.The monosaccharide compostion of MPS was composed mainly of galactose,glucose,rhamnose,arabinose,xylose and mannose and so on.APTT,PT and TT in vitro tests showed that they prolonged APTT and TT,but had no significant effect on PT.The result confirmed these in the regulation of coagulation initiated via the intrinsic pathway or common pathway;MPS-2b significantly prolonged APTT and TT compared to saline in experiment concentrations.These results suggest that the mulberry polysaccharide MPS-2b can be explored as a kind of natural potential anticoagulant.关键词
桑叶多糖/分离/纯化/抗凝血活性Key words
mulberry polysaccharides/isolation/purification/anticoagulant activity分类
生物科学引用本文复制引用
张入飞,蔡为荣,谢亮亮,杨蕾,陈俏..桑叶多糖的分离纯化及其抗凝血活性的研究[J].安徽工程大学学报,2015,(2):29-33,5.基金项目
国家自然科学基金资助项目(31171753) （31171753）
安徽省自然科学基金资助项目(1408085MC52) （1408085MC52）
芜湖市科技局资助项目(2013119) （2013119）
国家级大学生创新训练基金资助项目(201210363018) （201210363018）
