现代农业科技Issue(10)：77-78,84,3.
南药巴戟天毛状根诱导条件优化研究
Optimization of Inducement Conditions for Hairy Roots of Morinda officinalis How
摘要
Abstract
To establish the inducement conditions for hairy roots of Morinda officinalis How,A grobacterium rhizogene strains of ATCC15834,AR10,Msu440 were selected to induce the transformation ration of explants of buds,leaf,leafstalk and stem of Morinda officinalis How aseptic seedling into hairy root.The effect of infection time and concentrations of acetosyringone(AS)on the transformation ratio were also studied in this paper.The results showed that the better conditions for inducing hairy roots was as followsyoung buds as explant,infected by strain ATCC15834 for 20 min and then cultured in MS with 300μmol/L acetosyringone,in this conditions,the inducting rate reached 36.7%.关键词
巴戟天/毛状根诱导/发根农杆菌Key words
Morinda officinalis How/hairy roots inducement/Agrobacterium rhizogene分类
农业科技引用本文复制引用
郑传进,吴小勇,王志江..南药巴戟天毛状根诱导条件优化研究[J].现代农业科技,2014,(10):77-78,84,3.基金项目
广东省中医药局基金（20111251）。 （）
