现代农业科技Issue(12)：276-278,3.
超高效液相串联质谱法分析猪肉中残留的15种β-受体激动剂
Determination of 15 β-agonists Residues in Pork by Ultra-performance Liquid Chromatography-mass Spectrometry
摘要
Abstract
Objective]To develop a method for determination of 15β-agonists residues in pork by ultra-performance liquid chromatography-mass spectrometry.[Method]The samples were enzyme hydrolyzed byβ-Glucuronidase/aryl sulfatase,etracted with 0.2 mol/L Acetic acid-ammonium acetate buffering solution(pH=5.0),and cleaned up on a SPE cartridge,separated on a C18 column using 0.01mol/L ammonium acetate(contain 0.1%Formic Acid)-Methanol as mobile phase,and detected under multiple reaction monitoring(MRM)mode.[Results]The resultes displayed a good linearity for 15β-agonists over the range of 5~100 ng/mL.The mean recoveries were from 74.2%to 115.6%at three different concentrations.[Conclusion]The method was precise,sensitive,and highly efficient in extraction and purification,and it is suitable for fast analysis ofβ-agonists residues in a large number of pork samples.关键词
超高效液相色谱-串联质谱/猪肉/β-受体激动剂/固相萃取Key words
ultra-performance liquid chromatography-mass spectrometry/pork/β-agonists/solid phase extraction分类
轻工纺织引用本文复制引用
欧贝丽,倪伟华,邹燕,赵佳丽..超高效液相串联质谱法分析猪肉中残留的15种β-受体激动剂[J].现代农业科技,2015,(12):276-278,3.基金项目
浙江省科技厅分析测试科技计划项目（2013C37075）。 （）
