现代农业科技Issue(13)：186-187,2.
PnsICE1基因酵母双杂交诱饵载体构建及转录自激活检测
Construction of Yeast Two-Hybrid Bait Vector of PnsICE1 and Testing of Autonomous Transcriptional Activation
摘要
Abstract
In order to screen the PnsICE1 interacted proteins,the CDS sequence of PnsICE1 contains pGBKT7 vector homologous sequences was amplified using PCR and inserted into pGBKT7 vector to construct the bait vector for yeast two-hybrid(Y2H)system. Sequencing result showed that the PnsICE1 sequence was inserted into pGBKT7 vector in correct reading frame. Furthermore,the bait vector was transformed into Y2H yeast strain to test the autoactivation. The transformed yeast grew very well on SD/-Trp plate,in the SD/-Trp/X-a-Gal screening yeast,grown on the medium blue single colony,PnsICE1 transcription factor has the transcriptional activation activity.关键词
PnsICE1/诱饵载体/转录自激活/测序Key words
PnsICE1/bait vector/autoactivation/sequencing分类
农业科技引用本文复制引用
王艳敏..PnsICE1基因酵母双杂交诱饵载体构建及转录自激活检测[J].现代农业科技,2015,(13):186-187,2.基金项目
黑龙江省科研机构创新能力提升专项计划项目（YC2014D005）。 （）
