安徽医科大学学报Issue(5)：569-571,3.
缺血预处理对大鼠肢体缺血再灌注损伤保护效应的实验研究
Association between ischemic preconditioning time and the protective effects of hind limb ischemia/reperfusion injury in rats
摘要
Abstract
Objective To investigate the relationship between different ischemic preconditioning protocols and the protective effects of hind limb ischemia/reperfusion injury in rats in purpose of choosing an optimal ischemic pre-conditioning scheme. Methods An experimental study was designed using 40 SD rats divided in five groups ( n=8). Group A: the sham group, laparotomy and separating the abdominal aorta without clamping for 240 minutes ( min) . Group B:ischemia/reperfusion,rats submitted to ischemia for 120 min and reperfusion for 120 min. Group C, D and E:animals submitted to three cycles of clamping and releasing the aorta for 1, 5 and 10 min respectively before being submitted to the ischemia/reperfusion procedure; the oxidative damage, inflammatory and protective effects among five groups were evaluated by measuring serum levels of SOD, MDA, IL-6, TNF-α, IL-10. Results Compared with group B,SOD activities were significantly higher in group D(P<0.05),SOD activities were sig-nificantly higher in group E compared with group D(P<0.05). Compared with group B, the level of IL-6, TNF-α increased obviously in group C, D, E(P<0.05). IL-6, TNF-α, IL-10 was the highest in group E among five groups ( P <0.05 ) . Conclusion The relationship between ischemia preconditioning time and anti-oxidation in hind limb ischemia/reperfusion injury seems paralleled. The inflammatory response accompanied with the ischemia preconditioning becomes serious with ischemia time prolonging. Preconditioning doesn’t show anti-inflammatory effect. Five min/three circulation ischemia preconditioning scheme is an optimal protocol.关键词
大鼠/肢体/缺血再灌注/缺血预处理/保护机制Key words
rats/hind limb/ischemia/reperfusion/ischemic preconditioning/protective mechanism分类
医药卫生引用本文复制引用
司小毛,仇鹏,朱化刚,余康敏,查斌山,谢文涛,李俊..缺血预处理对大鼠肢体缺血再灌注损伤保护效应的实验研究[J].安徽医科大学学报,2014,(5):569-571,3.基金项目
安徽省卫生厅医学科研项目(编号:2010B17) （编号:2010B17）
