安徽医科大学学报Issue(5)：582-585,4.
二甲双胍对大鼠肾小球系膜细胞NF-资B、MCP-1、ICAM-1表达及合成的影响
Effects of metformin on expression and synthesis of intercellular adhesion molecule-1, monocyte chemoattractant protein-1 and nuclear factor-κB in rat glomerular mesangial cells
摘要
Abstract
Objective To observe the effects of metformin on expression and synthesis of nuclear factor-κB ( NF-κB), monocyte chemoattractant protein-1(MCP-1) and intercellular adhesion molecule-1(ICAM-1) in cultured rat glomerular mesangial cells( MCs) and explore its reno-protective mechanisms. Methods MCs were cultured in the medium with normal glucose ( group NG ) , high glucose ( group HG ) and different concentrations of metformin (group M1, M2, M3). After 48 h exposure, the supernatants and MCs were collected. The expression of NF-κB, MCP-1 and ICAM-1 mRNA was analyzed by Real-time PCR and MCP-1 protein synthesis by ELISA. NF-κBp65 and ICAM-1 were visualized by Western blot. Results ①MCs could express NF-κB, ICAM-1and MCP-1.②Af-ter being stimulated by high glucose, the levels of intracelluar NF-κB, MCP-1 and ICAM-1 were significantly in-creased compared with group NG(P<0.05). ③The levels of NF-κB, MCP-1 and ICAM-1 were significantly de-creased in group M1, M2 and group M3 compared with group HG(P<0.05). Conclusion Metformin can sup-press the expression and synthesis of NF-κB, MCP-1 and ICAM-1 of glomerular mesangial cells, which may partly contribute to its reno-protection.关键词
二甲双胍/核因子-κB/单核细胞趋化蛋白-1/细胞间黏附分子-1Key words
metformin/NF-κB/MCP-1/ICAM-1分类
医药卫生引用本文复制引用
顾俊菲,叶山东,汪姗,孙文佳,胡圆圆..二甲双胍对大鼠肾小球系膜细胞NF-资B、MCP-1、ICAM-1表达及合成的影响[J].安徽医科大学学报,2014,(5):582-585,4.基金项目
安徽省自然科学基金(编号:11040606M161) （编号:11040606M161）
安徽高校省级自然科学研究项目(编号:KJ2011A157) （编号:KJ2011A157）
